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文献和实验Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
in individual apoptotic cells by the in situ terminal deoxynucleotidyl transferase and nick translation assays. Cancer Res . 52: 1945. 3. Gorczyca, W.,Tuziak,T., Kram, A., Melamed, M.R., Darzynkiewicz, Z. 1994. Detection of apoptosis-associated DNA strand
Qualitative Detection of Apoptotic Cells Assessed by DNA Fragmentation
fragmentation and its ability to detect large DNA fragments (50 kb and 300–1000 kb) as opposed to the internucleosomal DNA ladder. It is a qualitative technique although it can be of limited quantitative value. It can be used to detect apoptosis in cell lines
The Detection of Specific DNA Sequences by Enhanced Chemiluminescence
Blotting transfer techniques are well-established procedures for the immobilization of DNA onto solid matrices, typically nitrocellulose or nylon membranes. The Southern blotting technique (ref. 1 and Chapter 15 ) has found many research
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