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- 保存条件:
2-8 °C
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- 抗体英文名:
Rabbit anti-Goat IgG (H&L), F(ab)'2 Fragment - Affinity Pure, Biotin Conjugate
- 抗体名:
Rabbit anti-Goat IgG (H&L), F(ab)'2 Fragment - Affinity Pure, Biotin Conjugate
- 规格:
0.5 mg
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文献和实验Anti-GFP/BrdU In Situ Immunostaining
IgG (Jackson ImmunoResearch, 111-096-006) Biotinylated anti-BrdU (AbCam, ab2284-125) Streptavidin-Alexa Flour 555 (Invitrogen/Molecular Probes, S21381) Goat serum Horse serum BSA 2N HCl DAPI Fluorescent mounting medium (Vectashield
do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10 the amount of antibody compared with other subclasses. In case of IgG1 subclass, add 3.3 M NaCl (192.85 g/L) to crude antibody
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
at room temperature or overnight at 4°C with gentle shaking. Drain off excess solution. 27. Add 40 µL of diluted affinity-purified primary antibody to each slide, and cover with a coverslip. Incubate the slides for 1 h at room
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