A synthetic peptide corresponding to a sequence at the N-terminus of human IRAK4(45-59aa DDRYNQFHIRRFEAL), identical to the related rat and mouse sequences.
内容
500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
IRAK-4(interleukin-1 receptor-associated kinase 4), also called REN64, in the IRAK family, is a protein kinase involved in signaling innate immune responses from Toll-like receptors. It also supports signaling from T-cell receptors. Scott(2002) mapped the REN64/IRAK4 gene to chromosome 12 based on similarity between the REN64 sequence(GenBank AF155118) and a chromosome 12 clone(GenBank AC093012). Functional analysis by Li et al.(2002) determined that IRAK4, like IRAK1 and Pelle, has auto- and cross-phosphorylation kinase activity. Precipitation and binding analyses showed weak interaction between IRAK4 and IRAK1, but IRAK4 did not interact with other IRAK family members. Overexpressed IRAK4 interacted with MYD88 and TRAF6 and activated mitogen-activated protein kinase(MAPK) and nuclear factor kappa-B(NFKB) pathways.
研究类别
1. Day, N., Tangsinmankong, N., Ochs, H., Rucker, R., Picard, C., Casanova, J.-L., Haraguchi, S., Good, R. Interleukin receptor-associated kinase (IRAK-4) deficiency associated with bacterial infections and failure to sustain antibody responses. J. Pediat. 144: 524-526, 2004. 2. Li, S., Strelow, A., Fontana, E. J., Wesche, H. IRAK-4: a novel member of the IRAK family with the properties of an IRAK-kinase. Proc. Nat. Acad. Sci. 99: 5567-5572, 2002. 3. Scott, A. F. Personal Communication. Baltimore, Md. 4/25/2002.
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。