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E.coli-derived human CYP1A2 recombinant protein (Position: H185-D320). Human CYP1A2 shares 69.9% and 72.1% amino acid (aa) sequence identity with mouse and rat CYP1A2, respectively.
Rabbit IgG
Lyophilized
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
单克隆
Human,Mouse,Rat
Rabbit
现货
博士德生物
WB,IHC-P
Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat;
100ul/200ul
规格: | 100ul | 产品价格: | ¥680.0 |
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规格: | 200ul | 产品价格: | ¥1180.0 |
货号 | PB0574 |
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产品名称 | ANTI-CYP1A2 Antibody |
基因名 | CYP1A2 |
抗体来源 | Rabbit |
克隆 | Polyclonal |
抗体亚型 | Rabbit IgG |
分子量 | 58KD |
免疫原 | E.coli-derived human CYP1A2 recombinant protein (Position: H185-D320). Human CYP1A2 shares 69.9% and 72.1% amino acid (aa) sequence identity with mouse and rat CYP1A2, respectively. |
内容 | 500ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA |
纯化方式 | Immunogen affinity purified. |
浓度 | Concentration: 0.1-0.5μg/ml; Tested Species: Human, Mouse, Rat; |
产品形态 | Lyophilized |
保存条件 | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing. |
背景资料 | CYP1A2 (Cytochrome P450, Subfamily I, Polypeptide 2) is a member of the cytochrome P450 mixed-function oxidase system and is involved in the metabolism of xenobiotics in the body. CYP1A2 is a member of the cytochrome P450 superfamily of enzymes. In humans, the CYP1A2 enzyme is encoded by the CYP1A2 gene. CYP1A2 localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The CYP1A2 gene encodes a P450 enzyme involved in O-deethylation of phenacetin. Ikeya et al. (1989) found that the human CYP1A2 gene spans almost 7.8 kb and contains 7 exons. The CYP1A2 gene is mapped on 15q24.1. CYP1A2 accounts for nearly 15% of the cytochrome P450 in the human liver. CYP1A2 displays higher activity in men than in women, and is inhibited by oral contraceptives. Inducers of CYP1A2 include cruciferous vegetables. Cigarette smoking has also been shown to increase CYP1A2 activity. Expression of CYP1A2 appears to be induced by various dietary constituents. |
研究类别 | 1. Browning, S. L., Tarekegn, A., Bekele, E., Bradman, N., Thomas, M. G. CYP1A2 is more variable than previously thought: a genomic biography of the gene behind the human drug-metabolizing enzyme. Pharmacogenet. Genomics 20: 647-664, 2010.2. Ikeya, K., Jaiswal, A. K., Owens, R. A., Jones, J. E., Nebert, D. W., Kimura, S. Human CYP1A2: sequence, gene structure, comparison with the mouse and rat orthologous gene, and differences in liver 1A2 mRNA expression. Molec. Endocr. 3: 1399-1408, 1989. 3. Nakajima, M., Yokoi, T., Mizutani, M., Kinoshita, M., Funayama, M., Kamataki, T. Genetic polymorphism in the 5-prime-flanking region of human CYP1A2 gene: effect on theCYP1A2 inducibility in humans. J. Biochem. 125: 803-808, 1999. |
Uniprot ID | CYP1A2: P05177 |
推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
为了提供最优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
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Western blot: | 1:500-2000 |
Immunohistochemistry in paraffin section: | 1:50-400 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
*最优稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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