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A synthetic peptide corresponding to a sequence at the N-terminus of human ARHGEF1 (41-71aa EQNSQFQSLEQVKRRPAHLMALLQHVALQFE), different from the related mouse and rat sequences by one amino acid.
Rabbit IgG
Lyophilized
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
单克隆
Human,Mouse,Rat
Rabbit
现货
博士德生物
WB,IHC-P,IHC-F,ICC/IF,FCM
Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat
100ul/200ul
规格: | 100ul | 产品价格: | ¥680.0 |
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规格: | 200ul | 产品价格: | ¥1180.0 |
货号 | PB1099 |
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产品名称 | ANTI-ARHGEF1 Antibody |
基因名 | ARHGEF1 |
抗体来源 | Rabbit |
克隆 | Polyclonal |
抗体亚型 | Rabbit IgG |
免疫原 | A synthetic peptide corresponding to a sequence at the N-terminus of human ARHGEF1 (41-71aa EQNSQFQSLEQVKRRPAHLMALLQHVALQFE), different from the related mouse and rat sequences by one amino acid. |
内容 | 500ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA |
纯化方式 | Immunogen affinity purified. |
浓度 | Concentration: 0.1-0.5μg/ml; Tested Species: Human, Rat |
产品形态 | Lyophilized |
保存条件 | At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing. |
背景资料 | Rho guanine nucleotide exchange factor 1 is a protein that in humans is encoded by the ARHGEF1 gene. Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. |
研究类别 | 1. Aasheim HC, Pedeutour F, Smeland EB (May 1997). "Characterization, expression and chromosomal localization of a human gene homologous to the mouse Lsc oncogene, with strongest expression in hematopoetic tissues". Oncogene 14 (14): 1747–52.2. Hart MJ, Sharma S, elMasry N, Qiu RG, McCabe P, Polakis P, Bollag G (Nov 1996). "Identification of a novel guanine nucleotide exchange factor for the Rho GTPase". J Biol Chem 271 (41): 25452–8. |
Uniprot ID | ARHGEF1: Q92888 |
推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
为了提供最优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
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Western blot: | 1:500-2000 |
Immunohistochemistry in paraffin section: | 1:50-400 |
Immunohistochemistry in frozen section: | 1:50-400 |
Immunocytochemistry in fixed cells: | 1:50-400 |
Flow Cytometry: | 1-3μg/1x106 cells |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
*最优稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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