200ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA and 50% glycerol.
纯化方式
Ascites
浓度
200ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
The tropomyosins are a family of actin filament binding proteins. These proteins were first isolated from skeletal muscle, but later identified in many nonmuscle tissues. Tropomyosins are ubiquitous proteins of 35 to 45 kD associated with the actin filaments of myofibrils and stress fibers. Vertebrates have at least 4 different tropomyosin genes; in humans, they are named TPM1, TPM2, TPM3, and TPM4. Tropomyosins expressed as different isoforms in muscle and non-muscle cells.
研究类别
1. Lees-Miller, J. P.; Helfman, D. M. : The molecular basis for tropomyosin isoform diversity. BioEssays 13: 429-437, 1991. 2. Gariboldi, M.; Manenti, G.; Dragani, T. A.; Pierotti, M. A. : Chromosome mapping of nine tropomyosin-related sequences in mice. Mammalian Genome 6: 273-277, 1995.
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
[list_product_images]Figure 1. Western blot analysis of anti- TPM1 antibody (BM1440). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat skeletal muscle tissue lysates,
Lane 2: rat heart tissue lysates,
Lane 3: mouse skeletal muscle tissue lysates,
Lane 4: mouse heart tissue lysates.
Use mouse anti- TPM1 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for TPM1 at approximately 35-45KD. The expected band size for TPM1 is at 33KD.[/list_product_images]