手机验证
0
0
0
0
单克隆
human
0
5-7天
博士德生物
IHC-P,FC,ELISA
0
200ul
产品名称 | TGFB1(TGFβ1) Antibody |
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产品描述 | Anti-TGFB1(TGFβ1) Antibody validated for IHC, FC, ELISA in Human. |
文献引用格式 | TGFB1(TGFβ1) Antibody (Boster Biological Technology, Wuhan, China. Catalog #BM0291) |
应用范围 | IHC, FC, ELISA |
抗体来源 | Mouse |
抗体亚型 | IgG1 |
免疫原 | Purified recombinant fragment of human TGFb1 (AA: 62-195) expressed in E. Coli. |
抗体规格 | 200ul/1180元 |
克隆 | Monoclonal |
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克隆号 | 单克隆抗体:IgG1;克隆号:7F6 |
产品形态 | Liquid |
内容 | Ascitic fluid containing 0.03% sodium azide. |
储存条件 | 连续使用时4℃存储,保质期六个月;长期存储时建议分装为10ul以上小包装-20℃存储,并避免反复冻融,保质期一年。 |
小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
基因名 | TGFB1 |
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基因全名 | Transforming growth factor beta-1 |
蛋白功能 | Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Colocalizes with ASPN in chondrocytes within OA lesions of articular cartilage. . |
Tissue Specificity | Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T- helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells. . |
Sequence Similarities | Transforming growth factor beta-1;TGF-beta-1;Latency-associated peptide;LAP;TGFB1;TGFB; |
Uniprot ID | TGFB1: P01137 |
This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease
为了提供最优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
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Western blotting | 1:100-400 |
Immunohistochemistry in paraffin section | 1:50-200 |
Immunocytochemistry in fixed cells | 1:50-200 |
Flow Cytometry | 1:40-80 |
enzyme-linked immuno sorbent assay | 1:2000 |
*最优稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Immunohistochemical analysis of paraffin-embedded lung cancer tissues using TGFb1 mouse mAb with DAB staining. |Immunohistochemical analysis of paraffin-embedded lymphoid tissue tissues using TGFb1 mouse mAb with DAB staining. |Flow cytometric analysis of A549 cells using TGFb1 mouse mAb (green) and negative control (red). |Black line: Control Antigen (100 ng);Purple line: Antigen(10ng);Blue line: Antigen (50 ng);Red line: Antigen (100 ng);[/list_product_images]
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