A synthetic peptide corresponding to a sequence at the N-terminus of human Sonic Hedgehog(203-218aa ATVHLEQGGTKLVKDL), identical to the related rat and mouse sequences.
内容
500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
The mouse, chicken, and zebrafish Shh homologs are highly conserved. SHH expression was not detected in adult tissues examined. However, it was expressed in fetal intestine, liver, lung, and kidney. SHH gene is mapped to 7q. SHH mutations are not a frequent cause of isolated oral clefts.
研究类别
1. Tsukurov, O.; Boehmer, A.; Flynn, J.; Nicolai, J.-P.; Hamel, B. C. J.; Traill, S.; Zaleske, D.; Mankin, H. J.; Yeon, H.; Ho, C.; Tabin, C.; Seidman, J. G.; Seidman, C. : A complex bilateral polysyndactyly disease locus maps to chromosome 7q36. Nature Genet. 6: 282-286, 1994.
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.
*最佳稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Lane 1: Rat Liver Tissue LysateLane 2: Rat Intestine Tissue LysateLane 3: HELA Cell LysateLane 4: A549 Cell LysateLane 5: SMMC Cell LysateLane 6: MM231 Cell Lysate|IHC(P): Rat Brain Tissue|IHC(P): Human Mammary Tissue[/list_product_images]