手机验证
0
0
0
0
单克隆
human
0
5-7天
博士德生物
WB,IC,FC,ELISA
0
200ul
产品名称 | RPS6KA3(RSK2) Antibody |
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产品描述 | Anti-RPS6KA3(RSK2) Antibody validated for WB, FC, ELISA in Human. |
文献引用格式 | RPS6KA3(RSK2) Antibody (Boster Biological Technology, Wuhan, China. Catalog #BM2559) |
应用范围 | WB, FC, ELISA |
抗体来源 | Mouse |
抗体亚型 | IgG1 |
免疫原 | Purified recombinant fragment of human RSK2 expressed in E. Coli. |
抗体规格 | 200ul/1180元 |
克隆 | Monoclonal |
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克隆号 | 单克隆抗体:IgG1;克隆号:4E10 |
产品形态 | Liquid |
内容 | Ascitic fluid containing 0.03% sodium azide. |
储存条件 | 连续使用时4℃存储,保质期六个月;长期存储时建议分装为10ul以上小包装-20℃存储,并避免反复冻融,保质期一年。 |
小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
基因名 | RPS6KA3 |
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基因全名 | Ribosomal protein S6 kinase alpha-3 |
蛋白功能 | Expressed in many tissues, highest levels in skeletal muscle. |
Tissue Specificity | Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1 and histone H3 at 'Ser-10', which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR- independent mechanism and promotes translation initiation by facilitating assembly of the preinitiation complex. In response to insulin, phosphorylates EIF4B, enhancing EIF4B affinity for the EIF3 complex and stimulating cap-dependent translation. Is involved in the mTOR nutrient-sensing pathway by directly phosphorylating TSC2 at 'Ser-1798', which potently inhibits TSC2 ability to suppress mTOR signaling, and mediates phosphorylation of RPTOR, which regulates mTORC1 activity and may promote rapamycin-sensitive signaling independently of the PI3K/AKT pathway. Mediates cell survival by phosphorylating the pro- apoptotic proteins BAD and DAPK1 and suppressing their pro- apoptotic function. Promotes the survival of hepatic stellate cells by phosphorylating CEBPB in response to the hepatotoxin carbon tetrachloride (CCl4). Is involved in cell cycle regulation by phosphorylating the CDK inhibitor CDKN1B, which promotes CDKN1B association with 14-3-3 proteins and prevents its translocation to the nucleus and inhibition of G1 progression. In LPS-stimulated dendritic cells, is involved in TLR4-induced macropinocytosis, and in myeloma cells, acts as effector of FGFR3-mediated transformation signaling, after direct phosphorylation at Tyr-529 by FGFR3. Negatively regulates EGF-induced MAPK1/3 phosphorylation via phosphorylation of SOS1. Phosphorylates SOS1 at 'Ser-1134' and 'Ser-1161' that create YWHAB and YWHAE binding sites and which contribute to the negative regulation of MAPK1/3 phosphorylation (By similarity). . |
Sequence Similarities | Ribosomal protein S6 kinase alpha-3;S6K-alpha-3;2.7.11.1;90 kDa ribosomal protein S6 kinase 3;p90-RSK 3;p90RSK3;Insulin-stimulated protein kinase 1;ISPK-1;MAP kinase-activated protein kinase 1b;MAPK-activated protein kinase 1b;MAPKAP kinase 1b;MAPKAPK-1b;Ribosomal S6 kinase 2;RSK-2;pp90RSK2;RPS6KA3;ISPK1, MAPKAPK1B, RSK2; |
Uniprot ID | RPS6KA3: P51812 |
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS).
为了提供最优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
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Western blotting | 1:100-400 |
Immunohistochemistry in paraffin section | 1:50-200 |
Immunocytochemistry in fixed cells | 1:50-200 |
Flow Cytometry | 1:40-80 |
enzyme-linked immuno sorbent assay | 1:2000 |
*最优稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]RSK2(BM2559)(MW:80KD)MCF-7细胞裂解,免疫印迹分析。[/list_product_images]
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