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发货周期:现货
博士德生物
cell culture supernates, serum, plasma(heparin) and urine.
96T
货号 | EK1542 |
---|---|
产品名称 | Human LDLR ELISA Kit |
产品描述 | For quantitative detection of human LDLR in cell culture supernates, serum, plasma(heparin) and urine. |
免疫原 | NSO, A22-R788 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <12pg/ml |
检测范围 | 62.5pg/ml-4000pg/ml |
基因名称 | LDLR |
基因全名 | low density lipoprotein receptor |
Uniprot ID | P01130 |
指标别名 | FH | FHC | LDL R | LDLR | LDL receptor | LDLCQ2 | P01130 |
背景资料 | The Low-Density Lipoprotein (LDL) Receptor is a mosaic protein of 839 amino acids (after removal of 21-amino acid signal peptide) that mediates the endocytosis of cholesterol-rich LDL. In humans, the LDL receptor protein is encoded by the LDLR gene. It belongs to the Low density lipoprotein receptor gene family. The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1542-CAP | 96-well plate precoated with anti- human LDLR antibody | 1 |
EK1542-ST | Lyophilized recombinant human LDLR standard | 10ng/tube×2 |
EK1542-DA | Biotinylated anti- human LDLR antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 99 | 718 | 1395 | 99 | 692 | 1264 |
Standard deviation | 5.94 | 39.49 | 93.46 | 6.43 | 39.44 | 97.32 |
CV(%) | 6% | 5.5% | 6.7% | 6.5% | 5.7% | 7.7% |
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.065 | 0.126 | 0.166 | 0.263 | 0.455 | 0.780 | 1.405 | 2.188 |
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