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发货周期:现货
博士德生物
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
96T
货号 | EK1500 |
---|---|
产品名称 | Human EDIL3 ELISA Kit |
产品描述 | For quantitative detection of human EDIL3 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, D24-E480 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | EDIL3 |
基因全名 | EGF like repeats and discoidin domains 3 |
Uniprot ID | O43854 |
指标别名 | DEL1 | Developmental endothelial locus 1 | Edil3 | Integrin binding protein DEL1 | Integrin-binding protein DEL1 | O43854 |
背景资料 | EDIL3, also known as DEL1, is a glycoprotein that is secreted by endothelial cells and can associate with the endothelial cell surface and the extracellular matrix. It is regulated by hypoxia or vascular injury and has been implicated in vascular remodeling during angiogenesis. The EDIL3 gene is mapped to 5q14. Human endothelial cells bind to Del1 together with the alpha-V (ITGAV)/beta-3 (ITGB3) integrin receptor. It is confirmed that adhesion of endothelial cells to Del1 is integrin mediated and that Del1 binds ITGAV/ITGB3. Functional analysis showed that both Del1 and vitronectin (VTN) stimulate endothelial cell migration. Immunoprecipitation and immunoblot analyses revealed that adhesion of endothelial cells to Del1 induces tyrosine phosphorylation of PTK2. |
显色时间 | 20-25分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1500-CAP | 96-well plate precoated with anti- human EDIL3 antibody | 1 |
EK1500-ST | Lyophilized recombinant human EDIL3 standard | 10ng/tube×2 |
EK1500-DA | Biotinylated anti- human EDIL3 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 39 | 255 | 385 | 39 | 236 | 411 |
Standard deviation | 1.95 | 14.53 | 26.95 | 2.3 | 16.28 | 31.64 |
CV(%) | 5% | 5.7% | 7% | 5.9% | 6.9% | 7.7% |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.014 | 0.056 | 0.107 | 0.175 | 0.353 | 0.779 | 1.391 | 1.954 |
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