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- Biorbyt
- 英国
- orb219885
- 2026年08月07日
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- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
12 months from date of receipt.
- 英文名：
DAPI Staining Kit
- 库存：
99
- 供应商：
Biorbyt
- 规格：
100 ul
描述: DAPI (4',6-diamidino-2-phenylindole) is a blue-fluorescent DNA stain that exhibits -20-fold enhancement of fluorescence upon binding to AT regions of dsDNA.It is used extensively in fluorescence microscopy. As DAPI can pass through an intact cell membrane, it can be used to stain both live and fixed cells, though it passes through the membrane less efficiently in live cells and therefore the effectiveness of the stain is lower.
应用: ICC, IF
保存条件: Store at -20°C for one year.
备注: For research use only.
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文献和实验DAPI Staining To visualize DNA, incubate fixed samples with 100 ng/ml 4',6'-diamidino-2-phenylindole hydrochloride (DAPI) in PBS for 30 min. Rinse 3x with PBTw. Materials PBS: Sambrook et al. (Molecular Cloning ). PBTw
Cell Cycle Staining Protocol-DAPI
in .5-2.0ml DAPI stain solution (final concentration of 1x106 cells/ml) and incubate for 30 min at 4degrees or on ice. DAPI Stain Solution 0.1%TritonX 100 in 10ml PBS add 100ul of 1mg/ml DAPI to the 10ml TritonX solution
DAPI Staining and Fluorescence Microscopy Techniques for Phytoplasmas
The 4′,6-diamidino-2-phenylindole (DAPI) stain technique is a simple method that was developed for confirming the presence of phytoplasmas in hand-cut or freezing microtome sections of infected tissues. DAPI binds AT-rich DNA preferentially
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