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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Mouse Human IgG Fab - Biotin Antibody抗体
- 抗体英文名:
Mouse Human IgG Fab - Biotin Antibody
- 应用范围:
ELISA, ICC, IHC, WB
- 宿主:
Mouse
- 适应物种:
Human
- 保质期:
6-12个月
- 抗原来源:
详询
- 目录编号:
orb216340
- 级别:
科研级
- 库存:
88
- 供应商:
biorbyt
- 标记物:
Biotin
- 克隆性:
Monoclonal
- 形态:
1 mg/ml. Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% rAlbumin, 50% glycerol, 0.05% Sodium Azide
- 免疫原:
Human IgG Fab
- 规格:
100 ul
免疫原:Human IgG Fab
应用稀释比例:ELISA: 1:5000-1:20000, WB: 1:5000-1:20000, IHC-P: 1:500-1:1000
纯化:The antibody was isolated from ascitic by immunoaffinity chromatography using antigens coupled to agarose beads.
保存说明:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
Note:For research use only.
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文献和实验CD20抗体Fab’片段,并在大肠杆菌中高效可溶性分泌表达[ 8 ] ,本研究的目的在于研究嵌合抗CD20Fab’片段的抗肿瘤活性及其作用机制。一、材料和方法1、材料MTT购自Sigma公司;Annexin-V为宝灵曼公司试剂盒;TRIZOL RNA提取试剂盒为美国Gibco公司产品;兔抗人bcl-2单克隆抗体和辣根过氧化物酶标记的羊抗兔IgG为北京中山生物技术有限公司产品,嵌合抗CD20Fab’片段为我室表达并纯化。β-actin引物(上游:5-TCATGTTTGAGACCTTCAA-3,下游
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
Immunohistochemisty-Fluorescence Protocol
antibody (either biotin-donkey anti-goat IgG Fab2 diluted 1:700 or biotin-goat anti-mouse IgG1 or IgG2A or IgG2B diluted 1:500) in Wash Buffer-Saponin. Wash the cells twice in Wash Buffer-Saponin. Incubate the centrifuged cell pellet
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