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Cytokeratin 16 Antibody抗体，orb2

14170，biorbyt
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  • ￥1560
  • biorbyt已认证
  • orb214170
  • 英国
  • 2025年12月04日
  • IF, IHC, WB
  • Rabbit
  • Human, Mouse, Rat
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武汉博欧特生物科技有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名：

      Cytokeratin 16 Antibody抗体

    • 抗体英文名：

      Cytokeratin 16 Antibody

    • 应用范围：

      IF, IHC, WB

    • 宿主：

      Rabbit

    • 适应物种：

      Human, Mouse, Rat

    • 保质期：

      6-12个月

    • 抗原来源：

      详询

    • 目录编号：

      orb214170

    • 级别：

      科研级

    • 库存：

      88

    • 供应商：

      biorbyt

    • 标记物：

      Unconjugated

    • 克隆性：

      Polyclonal

    • 形态：

      Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

    • 免疫原：

      KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cytokeratin 16. The exact sequence is proprietary.

    • 规格：

      30 ul

    产品描述：The Cytokeratin 16 Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cytokeratin 16. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

    别名:KRT16A; Keratin, type I cytoskeletal 16; Cytokeratin-16; CK-16; Keratin-16; K16

    免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Cytokeratin 16. The exact sequence is proprietary.

    应用稀释比例：WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500

    纯化:The antibody was purified by immunogen affinity chromatography.

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Entrez:3868, 16666

    UniProt ID:P08779, Q9Z2K1

    Note:For research use only.

    产品细节图片1
    Western blot analysis of Cytokeratin 16 expression in H446 (A), mouse lung (B), mouse brain (C), rat brain (D) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 51 kD)

    产品细节图片2
    Immunohistochemical analysis of Cytokeratin 16 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    产品细节图片3
    Immunofluorescent analysis of Cytokeratin 16 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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