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14-3-3 zeta (Phospho-S58) Anti

body抗体,orb214750,biorbyt
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  • ¥1820
  • biorbyt已认证
  • orb214750
  • 英国
  • 2025年12月04日
  • IF, IHC, WB
  • Rabbit
  • Bovine, Gallus, Human, Mouse, Porcine, Rat, Sheep, Zebrafish
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      14-3-3 zeta (Phospho-S58) Antibody抗体

    • 抗体英文名

      14-3-3 zeta (Phospho-S58) Antibody

    • 应用范围

      IF, IHC, WB

    • 宿主

      Rabbit

    • 适应物种

      Bovine, Gallus, Human, Mouse, Porcine, Rat, Sheep, Zebrafish

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb214750

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

    • 免疫原

      KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S58 of human 14-3-3 zeta protein. The exact sequence is proprietary.

    • 规格

      30 ul

    产品描述:The 14-3-3 zeta (Phospho-S58) Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S58 of human 14-3-3 zeta protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

    别名:14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1

    免疫原:KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S58 of human 14-3-3 zeta protein. The exact sequence is proprietary.

    应用稀释比例:WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500, IP: 1-10-1-100

    纯化:The antibody was purified by immunogen affinity chromatography.

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Entrez:25578, 22631, 7534

    UniProt ID:P63101, P63102, P63104

    Note:For research use only.

    产品细节图片1
    Western blot analysis of 14-3-3 zeta (Phospho-S58) expression in MCF7 (A), Jurkat (B), mouse brain (C), rat brain (D) whole cell lysates. (Predicted band size: 27 kD; Observed band size: 28 kD)

    产品细节图片2
    Immunohistochemical analysis of 14-3-3 zeta (Phospho-S58) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    产品细节图片3
    Immunofluorescent analysis of 14-3-3 zeta (Phospho-S58) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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    图标文献和实验
    相关实验
    • 14-3-3 proteins

      The human and bovine 14-3-3 eta protein mRNAs are highly conservedEvolutionary conservation of the 14-3-3 proteinExpression and structural analysis of 14-3-3 proteinsCrystal structure of the zeta isoform of the 14-3-3 proteinStructure of a 14

    • Introduction of human 14-3-3 proteins

      terminus of GPIb-alpha,as indicated by antibody inhibition and direct binding of 14-3-3zeta to synthetic GPIb-alpha cytoplasmic domain peptides.The 14-3-3zeta binds to recombinant wild type GPIb-IX but not to the GPIb-alpha mutants lacking C-terminal

    • 实验达人推荐的磷酸化蛋白的Western操作指南

      相关专题   在western blot ting 方面,本文绝对是一个新手,目前正准备磷酸化蛋白的Western,急需这方面的实验技术操作步骤和一些注意事项的指导。在网络上搜集整理了一个western blot实验达人的建议,供大家参考。 我做了很久的western blot ,尤其是phospho-抗体,可以算是达人了。我根据自己的经验,对磷酸化蛋白之western blot提出以下建议,供刚入门的同仁参考

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