- ￥1560
- biorbyt
- orb214600
- 英国
- 2025年12月04日
- IF, WB
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
SOS2 Antibody抗体
- 抗体英文名：
SOS2 Antibody
- 应用范围：
IF, WB
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb214600
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 形态：
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
- 免疫原：
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SOS2. The exact sequence is proprietary.
- 规格：
30 ul
产品描述：The SOS2 Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SOS2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
别名:Son of sevenless homolog 2; SOS-2
免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SOS2. The exact sequence is proprietary.
应用稀释比例：WB: 1-500-1-1000
纯化:The antibody was purified by immunogen affinity chromatography.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Entrez:20663, 6655
UniProt ID:Q02384, Q07890
Note:For research use only.
别名:Son of sevenless homolog 2; SOS-2
免疫原：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SOS2. The exact sequence is proprietary.
应用稀释比例：WB: 1-500-1-1000
纯化:The antibody was purified by immunogen affinity chromatography.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Entrez:20663, 6655
UniProt ID:Q02384, Q07890
Note:For research use only.
Western blot analysis of SOS2 expression in A549 (A), SP2/0 (B), H9C2 (C) whole cell lysates. (Predicted band size: 152 kD; Observed band size: 153 kD)
Immunofluorescent analysis of SOS2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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文献和实验相关实验
SOS信号转导途径在植物离子平衡和耐盐中非常重要。SOS模型认为高Na+引起了胞内自由Ca2+的升高，激活了Ca2+结合蛋白编码的SOS3的表达，影响到下游的反应。SOS3激活了相连的SOS2（丝氨酸/苏氨酸蛋白激酶），SOS2/SOS3复合体调节盐忍耐因子编码的SOS1（质膜Na+/H+反向转运体）的表达水平，从而促使胞内过多的Na+排出体外。这其中涉及到K+的变化，Na+和K+平衡的破坏可能是SOS途径中的关键，但是详细的情况不清楚。尤其是在活细胞和组织水平的信号和离子转运缺乏电生理
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SOS2 Antibody抗体，orb214600，biorbyt
￥1560
