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Cytochrome P450 2J2 Antibody抗体

,orb213827,biorbyt
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  • ¥1560
  • biorbyt已认证
  • orb213827
  • 英国
  • 2025年12月04日
  • IF, IHC, WB
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      Cytochrome P450 2J2 Antibody抗体

    • 抗体英文名

      Cytochrome P450 2J2 Antibody

    • 应用范围

      IF, IHC, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb213827

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

    • 免疫原

      KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cytochrome P450 2J2. The exact sequence is proprietary.

    • 规格

      30 ul

    产品描述:The Cytochrome P450 2J2 Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cytochrome P450 2J2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

    别名:Cytochrome P450 2J2; Arachidonic acid epoxygenase; CYPIIJ2

    免疫原:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Cytochrome P450 2J2. The exact sequence is proprietary.

    应用稀释比例:WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500, IP: 1-10-1-100

    纯化:The antibody was purified by immunogen affinity chromatography.

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Entrez:1573

    UniProt ID:P51589

    Note:For research use only.

    产品细节图片1
    Western blot analysis of Cytochrome P450 2J2 expression in H1792 (A), MEF (B), mouse liver (C) whole cell lysates. (Predicted band size: 57 kD; Observed band size: 57 kD)

    产品细节图片2
    Immunohistochemical analysis of Cytochrome P450 2J2 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    产品细节图片3
    Immunofluorescent analysis of Cytochrome P450 2J2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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    图标文献和实验
    相关实验
    • Cytochrome P450 Nomenclature

      With genome projects spewing forth DNA sequence at tens of Megabases per year, the problem of genetic nomenclature becomes daunting. In the field of cytochrome P450 (P450), there are more than 750 sequences and they are accumulating rapidly

    • Cytochrome P450 Nomenclature, 2004

      Aspects of cytochrome P450 (CYP) nomenclature are addressed. The rules for naming a P450 are outlined, though individuals should not name their own genes. The nomenclature is presented as a unifying principle to enhance communication

    • Genotyping for Cytochrome P450 Polymorphisms

      Protocols for the extraction of DNA from human blood and for genotyping for a number of common cytochrome P450 polymorphisms using either polymerase chain reaction (PCR)-restriction fragment length polymorphism or PCR-single-strand

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