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PI3K p85 alpha/p55 gamma Antib

ody抗体,orb214397,biorbyt
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  • ¥1560
  • biorbyt已认证
  • orb214397
  • 英国
  • 2025年12月04日
  • IF, IHC, WB
  • Rabbit
  • Bovine, Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      PI3K p85 alpha/p55 gamma Antibody抗体

    • 抗体英文名

      PI3K p85 alpha/p55 gamma Antibody

    • 应用范围

      IF, IHC, WB

    • 宿主

      Rabbit

    • 适应物种

      Bovine, Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb214397

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

    • 免疫原

      KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PI3K p85 alpha/p55 gamma. The exact sequence is proprietary.

    • 规格

      30 ul

    产品描述:The PI3K p85 alpha/p55 gamma Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PI3K p85 alpha/p55 gamma. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

    别名:PIK3R1; GRB1; Phosphatidylinositol 3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha; PIK3R3; Phosphatidylinositol 3-kinase regulatory subunit gamma; PI3-kinase regulatory subunit gamma; PI3K regulatory subunit gamma; PtdIns-3-kinase regulatory subunit gamma; Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; PI3-kinase subunit p55-gamma; PtdIns-3-kinase regulatory subunit p55-gamma; p55PIK

    免疫原:KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PI3K p85 alpha/p55 gamma. The exact sequence is proprietary.

    应用稀释比例:WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500

    纯化:The antibody was purified by immunogen affinity chromatography.

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Entrez:25513, 60664, 18710, 5295, 8503, 18708

    UniProt ID:Q63789, P27986, P26450, Q64143, Q63787, Q92569

    Note:For research use only.

    产品细节图片1
    Western blot analysis of PI3K p85 alpha/p55 gamma expression in HeLa (A), mouse brain (B), rat brain (C), rat muscle (D) whole cell lysates. (Predicted band size: 83; 54 kD; Observed band size: 85; 55 kD)

    产品细节图片2
    Immunohistochemical analysis of PI3K p85 alpha/p55 gamma staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    产品细节图片3
    Immunofluorescent analysis of PI3K p85 alpha/p55 gamma staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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      .    Lee, J.Y., Chiu, Y.H., Asara, J., and Cantley, L.C. 2011. Inhibition of PI3K binding to activators by serine phosphorylation of PI3K regulatory subunit p85alpha Src homology‐2 domains. Proc. Natl. Acad. Sci. U

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