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Calnexin Antibody抗体,orb213637,

biorbyt
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  • ¥1560
  • biorbyt已认证
  • orb213637
  • 英国
  • 2025年12月04日
  • IF, IHC, WB
  • Rabbit
  • Bovine, Canine, Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      Calnexin Antibody抗体

    • 抗体英文名

      Calnexin Antibody

    • 应用范围

      IF, IHC, WB

    • 宿主

      Rabbit

    • 适应物种

      Bovine, Canine, Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb213637

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

    • 免疫原

      KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Calnexin. The exact sequence is proprietary.

    • 规格

      30 ul

    产品描述:The Calnexin Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Calnexin. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

    别名:Calnexin; IP90; Major histocompatibility complex class I antigen-binding protein p88; p90

    免疫原:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Calnexin. The exact sequence is proprietary.

    应用稀释比例:WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500, IP: 1-10-1-100

    纯化:The antibody was purified by immunogen affinity chromatography.

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Entrez:29144, 12330, 821

    UniProt ID:P27824, P35565, P35564

    Note:For research use only.

    产品细节图片1
    Western blot analysis of Calnexin expression in Hela (A), mouse lung (B) whole cell lysates. (Predicted band size: 67 kD; Observed band size: 90 kD)

    产品细节图片2
    Immunohistochemical analysis of Calnexin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    产品细节图片3
    Immunofluorescent analysis of Calnexin staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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    图标文献和实验
    相关实验
    • In Vitro Assays of the Functions of Calnexin and Calreticulin, Lectin Chaperones of the Endoplasmic Reticulum

      Calnexin and calreticulin are molecular chaperones of the endoplasmic reticulum (ER) whose folding-promoting functions are directed predominantly toward aspargine-linked glycoproteins. This is a consequence of calnexin and calreticulin

    • 速看!脑组织分离外泌体终于出炉!

      101 和 Calnexin。WB 结果如下: 图三 WB 鉴定脑组织外泌体蛋白 marker 从 WB 结果可以看出,CD9 和 TSG101 在外泌体样本中呈现阳性结果,Calnexin 在外泌体样本中呈现阴性结果。符合外泌体的蛋白特征。 从以上三种常用的外泌体鉴定结果中可得出,本次使用的从小鼠脑组织中分离外泌体的方法是可行的,能顺利获得需要的外泌体。但是方法中也存在一些不足,从电镜结果中可以看出,样本中可能还有一些杂质掺杂。 如果要进一步除杂,可能就要面临损失很多外泌体的风险,所以经常遇到的问题

    • Analyzing Association of the Endoplasmic Reticulum with the Legionella pneumophilaContaining Vacuoles by Fluorescence Microscopy

      . The recruitment of YFP-KDEL, GFP-p58, calnexin, and myc-Sec22b to the L. pneumophila – containing vacuole can be assessed by fluorescence microscopy. Methods for detection of these various ER markers during infection of mammalian cells by L. pneumophila

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