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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Calnexin Antibody抗体
- 抗体英文名:
Calnexin Antibody
- 应用范围:
IF, IHC, WB
- 宿主:
Rabbit
- 适应物种:
Bovine, Canine, Human, Mouse, Rat
- 保质期:
6-12个月
- 抗原来源:
详询
- 目录编号:
orb213637
- 级别:
科研级
- 库存:
88
- 供应商:
biorbyt
- 标记物:
Unconjugated
- 克隆性:
Polyclonal
- 形态:
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
- 免疫原:
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Calnexin. The exact sequence is proprietary.
- 规格:
30 ul
别名:Calnexin; IP90; Major histocompatibility complex class I antigen-binding protein p88; p90
免疫原:KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Calnexin. The exact sequence is proprietary.
应用稀释比例:WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500, IP: 1-10-1-100
纯化:The antibody was purified by immunogen affinity chromatography.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Entrez:29144, 12330, 821
UniProt ID:P27824, P35565, P35564
Note:For research use only.



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文献和实验Calnexin and calreticulin are molecular chaperones of the endoplasmic reticulum (ER) whose folding-promoting functions are directed predominantly toward aspargine-linked glycoproteins. This is a consequence of calnexin and calreticulin
101 和 Calnexin。WB 结果如下: 图三 WB 鉴定脑组织外泌体蛋白 marker 从 WB 结果可以看出,CD9 和 TSG101 在外泌体样本中呈现阳性结果,Calnexin 在外泌体样本中呈现阴性结果。符合外泌体的蛋白特征。 从以上三种常用的外泌体鉴定结果中可得出,本次使用的从小鼠脑组织中分离外泌体的方法是可行的,能顺利获得需要的外泌体。但是方法中也存在一些不足,从电镜结果中可以看出,样本中可能还有一些杂质掺杂。 如果要进一步除杂,可能就要面临损失很多外泌体的风险,所以经常遇到的问题
. The recruitment of YFP-KDEL, GFP-p58, calnexin, and myc-Sec22b to the L. pneumophila – containing vacuole can be assessed by fluorescence microscopy. Methods for detection of these various ER markers during infection of mammalian cells by L. pneumophila
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