OTTHUMP00000016990,Wiskott-Aldrich syndrome protein family member 1,homology of dictyostelium scar 1,verprolin homology domain-containing protein 1
Gene Summary:
The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Gene Description:
WAS protein family, member 1
Gene Alias:
FLJ31482,KIAA0269,SCAR1,WAVE,WAVE1
Gene Name:
WASF1
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage Instruction:
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Storage Buffer:
In PBS (without Mg and Ca), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Recommend Usage:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:100) Western Blot (1:500-1:3000) The optimal working dilution should be determined by the end user.
Isotype:
IgG
Purification:
Affinity chromatography
Form:
Liquid
Specificity:
WASF1 (phospho Y125) polyclonal antibody detects endogenous levels of human WASF1 only when phosphorylated at tyrosine 125.
Reactivity:
Human, Mouse
Theoretical MW (kDa):
70
Host:
Rabbit
Immunogen:
A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y125 of human WASF1.
Product Description:
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human WASF1.