- 询价
- Cell Signaling Technology
- 9532
- USA
- 2025年07月15日
- W, IP, IF-IC
- H,M,R,Mk
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
PARP (46D11) Rabbit mAb
- 抗原：
synthetic peptide corresponding to residues surrounding Gly623 of PARP
- 应用范围：
W, IP, IF-IC
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 供应商：
CST
- 库存：
大量
- 适应物种：
H,M,R,Mk
- 是否单克隆：
多克隆
- 保存条件：
-20°c
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IF-IC | H M R Mk | Endogenous | 116, 89 | Rabbit IgG |
| Protocols | |
|---|---|
| Specificity / Sensitivity | PARP (46D11) Rabbit mAb detects endogenous levels of total full-length PARP and the large fragment (89 kDa) produced by caspase cleavage. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly623 of PARP. Western Blotting
Western blot analysis of extracts from THP-1 cells, untreated or treated with TNF-α and cycloheximide as well as control extracts from SW620 and A20 cell lines, using PARP (46D11) Rabbit mAb. IF-IC
Confocal immunofluorescent analysis of untreated HeLa cells labeled with PARP (46D11) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). |
| Background | PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).
|
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063. For Research Use Only. Not For Use In Diagnostic Procedures. |
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