手机验证
/
详见说明书
大量
CST
/
人,小鼠,大鼠,驴,细菌,猪,鸡,狗,马
western blot,免疫组化(IHC),免疫组化(IHC),免疫荧光(IF),流式细胞(Flow Cyt)
兔
β-Actin (13E5) Rabbit mAb
1
-20°c
详见MSDS文件
40 ul (4 western blots)/100 ul (10 western blots)/300 ul (30 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /40 ul (4 western blots)/100 ul (10 western blots)/300 ul (30 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
规格: | 产品价格: | ¥面议 | |
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规格: | 40 ul (4 western blots) | 产品价格: | ¥请询价 |
规格: | 100 ul (10 western blots) | 产品价格: | ¥请询价 |
规格: | 300 ul (30 western blots) | 产品价格: | ¥请询价 |
规格: | carrier free & custom formulation / quantity | 产品价格: | ¥请询价 |
规格: | 40 ul (4 western blots) | 产品价格: | ¥请询价 |
规格: | 100 ul (10 western blots) | 产品价格: | ¥请询价 |
规格: | 300 ul (30 western blots) | 产品价格: | ¥请询价 |
规格: | carrier free & custom formulation / quantity | 产品价格: | ¥请询价 |
PhosphoSitePlus® protein, site, and accession data: ACTB
Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
---|---|---|---|---|
W IHC-P IHC-F IF-IC F | H M R Mk B Pg (C) (Dg) (Hr) | Endogenous | 45 | Rabbit IgG |
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey C=Chicken B=Bovine Dg=Dog Pg=Pig Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
* Product-specific protocol.
β-Actin (13E5) Rabbit mAb detects endogenous levels of total β-actin protein. This antibody may cross-react with the γ-actin (cytoplasmic isoform). It does not cross-react with α-skeletal, α-cardiac, α-vascular smooth, or γ-enteric smooth muscle isoforms.
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino-terminus of human β-actin protein.
Western blot analysis of cell extracts from various cell lines using beta-Actin (13E5) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human heart using beta-Actin (13E5) Rabbit mAb. Note the lack of staining of cardiac actin.
Immunohistochemical analysis of paraffin-embedded human leiomyoma using beta-Actin (13E5) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mouse tumor using β-actin (13E5) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using beta-Actin (13E5) Rabbit mAb in the presence of control peptide (left) or beta-Actin Blocking Peptide #1025 (right).
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using beta-Actin (13E5) Rabbit mAb. Note the lack of staining of skeletal muscle actin.
Immunohistochemical analysis of frozen H1650 xenograft showing membrane and cytoplasmic localization using β-Actin (13E5) Rabbit mAb.
Flow cytometric analysis of NIH/3T3 cells using β-Actin (13E5) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
Confocal immunofluorescent analysis of COS-7 cells using β-Actin (13E5) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The Arp2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). Research studies have shown that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle, as shown in research studies (4-6). Actin cleavage by caspase-3 may accelerate ubiquitin/proteasome-dependent muscle proteolysis (6).
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
For Research Use Only. Not For Use In Diagnostic Procedures.
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