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Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor 488 Conjugate)
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流式细胞(Flow Cyt)
兔
详见MSDS文件
人,小鼠,大鼠,仓鼠,驴,猪,狗
CST
大量
/
详见说明书
1
-20°c
100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
规格: | 产品价格: | ¥面议 | |
---|---|---|---|
规格: | 100 ul (50 tests) | 产品价格: | ¥请询价 |
规格: | carrier free & custom formulation / quantity | 产品价格: | ¥请询价 |
规格: | 100 ul (50 tests) | 产品价格: | ¥请询价 |
规格: | carrier free & custom formulation / quantity | 产品价格: | ¥请询价 |
PhosphoSitePlus® protein, site, and accession data: NFkB-p65
Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
---|---|---|---|---|
F | H M R Hm Mk Pg (Dg) | Endogenous | 65 | Rabbit IgG |
Applications Key: F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey Dg=Dog Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor®488 Conjugate) detects NF-kappaB p65 only when phosphorylated at serine 536. It does not cross-react with the p50 subunit or other related proteins.
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser536 of human NF-kappaB p65. The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-6.
Cell Signaling Technology Antibody conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct Flow Cytometric analysis of human cells. The unconjugated antibody, #3033, reacts with Phospho-NF-KappaB p65 (Ser536) from human, mouse, and rat. CST expects that Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor® 488 Conjugate) will also recognize Phospho-NF-KappaB p65 (Ser536) in these species.
Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11).
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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
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