- 询价
- Cell Signaling Technology
- 4780
- USA
- 2025年07月16日
- 流式细胞（Flow Cyt）
- 兔
- 人,小鼠,大鼠,仓鼠,驴,其它,其他,斑马鱼,细菌,猪,线虫
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Alexa Fluor 488 Conjugate)
- 抗原：
/
- 应用范围：
流式细胞（Flow Cyt）
- 宿主：
兔
- 供应商：
CST
- 适应物种：
人,小鼠,大鼠,仓鼠,驴,其它,其他,斑马鱼,细菌,猪,线虫
- 抗原来源：
/
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 库存：
大量
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥面议 | |
|---|---|---|---|
| 规格： | 100 ul (50 tests) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
| 规格： | 100 ul (50 tests) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - MAPK Signaling
p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4780
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| F | H M R Hm Mk Mi Dm Z B Pg Ce | Endogenous | Rabbit IgG |
Applications Key: F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey Mi=Mink Dm=D. melanogaster Z=Zebrafish B=Bovine Pg=Pig Sc=S. cerevisiae Ce=C. elegans
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4780:
- Flow
Specificity / Sensitivity
p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total p44/42 MAPK protein. The antibody does not cross-react with JNK/SAPK or p38 MAP kinase.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of rat p44 MAP kinase. This antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-6.
Flow Cytometry
Flow cytometric analysis of Jurkat cells, using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green) compared to XP® Rabbit (DA1E) mAb IgG Isotype Control (Alexa Fluor® 488 Conjugate) #2975 (blue).
Description
This Cell Signaling Technology (CST) antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody (p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695).
Background
Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059.
- Roux, P.P. and Blenis, J. (2004) Microbiol Mol Biol Rev 68, 320-44.
- Baccarini, M. (2005) FEBS Lett 579, 3271-7.
- Meloche, S. and Pouysségur, J. (2007) Oncogene 26, 3227-39.
- Roberts, P.J. and Der, C.J. (2007) Oncogene 26, 3291-310.
- Rubinfeld, H. and Seger, R. (2005) Mol Biotechnol 31, 151-74.
- Murphy, L.O. and Blenis, J. (2006) Trends Biochem Sci 31, 268-75.
- Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
- Marais, R. et al. (1993) Cell 73, 381-93.
- Kortenjann, M. et al. (1994) Mol Cell Biol 14, 4815-24.
- Owens, D.M. and Keyse, S.M. (2007) Oncogene 26, 3203-13.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 4695 p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb
- 4284 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate)
- 4344 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate)
- 2975 Rabbit (DA1E) mAb IgG XP® Isotype Control (Alexa Fluor® 488 Conjugate)
Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063 and U.S.S.N. 11/476,277) from Epitomics, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
secondary antibody review -- data from 99 publications
ref host class subclass conjugation experiment dilution detail supplier 1 goat Alexa Fluor 488 immunocytochemistry 1:250 localization of tagged EVA-1 in 293T cell Molecular Probes 1 goat Alexa Fluor 546 immunocytochemistry 1:250
and ERK1/2 kinases.2.Materials and methods2.1.Chemicals and reagentsRoswell Park Memorial Institute 1640 (RPMI 1640),modified Eagle’s medium (MEM), fetal bovine serum (FBS), trypan blue solution and horseradish peroxidaseconjugated goat anti-rabbit
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