p44/42 MAPK (Erk1/2) (137F5) R

Product Pathways - MAPK Signaling

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p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IP IHC-P IF-IC F	H M R Hm Mk Mi Dm Z B Dg Pg Ce (C)	Endogenous	42, 44	Rabbit IgG

Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey C=Chicken Mi=Mink Dm=D. melanogaster Z=Zebrafish B=Bovine Dg=Dog Pg=Pig Ce=C. elegans
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

4695:

Flow , IHC / Paraffin , Immunofluorescence , Immunoprecipitation , Western Blotting

Specificity / Sensitivity

p44/42 MAP Kinase (137F5) Rabbit mAb detects endogenous levels of total p44/42 MAP kinase (Erk1/Erk2) protein. The antibody does not cross-react with JNK/SAPK or p38 MAP kinase.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the C-terminus of rat p44 MAP kinase.

Background

Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059.

1. Roux, P.P. and Blenis, J. (2004) Microbiol Mol Biol Rev 68, 320-44.
2. Baccarini, M. (2005) FEBS Lett 579, 3271-7.
3. Meloche, S. and Pouysségur, J. (2007) Oncogene 26, 3227-39.
4. Roberts, P.J. and Der, C.J. (2007) Oncogene 26, 3291-310.
5. Rubinfeld, H. and Seger, R. (2005) Mol Biotechnol 31, 151-74.
6. Murphy, L.O. and Blenis, J. (2006) Trends Biochem Sci 31, 268-75.
7. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
8. Marais, R. et al. (1993) Cell 73, 381-93.
9. Kortenjann, M. et al. (1994) Mol Cell Biol 14, 4815-24.
10. Owens, D.M. and Keyse, S.M. (2007) Oncogene 26, 3203-13.

Application References

• Yang, P. et al. (2010) Nat Immunol 11, 487-94. Applications: Western Blotting
• Kang, L. et al. (2010) Mol Endocrinol 24, 709-21. Applications: Western Blotting
• Rohani, M.G. et al. (2010) BMC Immunol 11, 53. Applications: Western Blotting
• Miyaji, M. et al. (2009) Proc Natl Acad Sci U S A 106, 14502-7. Applications: Western Blotting
• Dumesic, P.A. et al. (2009) J Cell Biol 185, 409-22. Applications: IHC-P (paraffin) Western Blotting
• Mabilleau, G. and Sabokbar, A. (2009) PLoS ONE 4, e4173. Applications: Western Blotting
• Gray, M.J. et al. (2008) J Natl Cancer Inst 100, 109-20. Applications: Western Blotting
• Rygiel, T.P. et al. (2008) J Cell Sci 121, 1183-92. Applications: Western Blotting
• Engelman, J.A. et al. (2008) Nat Med 14, 1351-6. Applications: Western Blotting
• Hyman, J.M. et al. (2009) Proc Natl Acad Sci U S A 106, 14132-7. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

Companion Products

• 9194 p44/42 MAPK (Erk1/2) Control Cell Extracts
• 4370 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP^® Rabbit mAb
• 4376 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit mAb
• 4377 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb
• 9101 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody
• 9106 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb
• 4372 p44 MAP Kinase (Erk1) Antibody
• 4696 p44/42 MAPK (Erk1/2) (L34F12) Mouse mAb
• 9102 p44/42 MAPK (Erk1/2) Antibody
• 9107 p44/42 MAPK (Erk1/2) (3A7) Mouse mAb
• 9108 p42 MAP Kinase (Erk2) Antibody
• 1240 p44/42 MAPK (Erk1/2) Blocking Peptide (#4695 Specific)
• 8103 SignalSlide^® Phospho-p44/42 MAPK (Thr202/Tyr204) IHC Controls
• 8112 SignalStain^® Antibody Diluent
• 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 8114 SignalStain^® Boost IHC Detection Reagent (HRP, Rabbit)

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

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For Research Use Only. Not For Use In Diagnostic Procedures.