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GAPDH (14C10) Rabbit mAb (Alexa Fluor 488 Conjugate)
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免疫荧光(IF),流式细胞(Flow Cyt)
兔
人,小鼠,大鼠,驴
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详见说明书
大量
详见MSDS文件
CST
1
-20°c
100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
规格: | 产品价格: | ¥面议 | |
---|---|---|---|
规格: | 100 ul (50 tests) | 产品价格: | ¥请询价 |
规格: | carrier free & custom formulation / quantity | 产品价格: | ¥请询价 |
规格: | 100 ul (50 tests) | 产品价格: | ¥请询价 |
规格: | carrier free & custom formulation / quantity | 产品价格: | ¥请询价 |
PhosphoSitePlus® protein, site, and accession data: GAPDH
Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
---|---|---|---|---|
IF-IC F | H M R Mk | Endogenous | 37 | Rabbit IgG |
Applications Key: IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
GAPDH (14C10) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total GAPDH protein.
Monoclonal antibody is produced by immunizing animals with a synthetic peptide near the carboxy terminus of human GAPDH. This antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-6.
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated GAPDH (14C10) Rabbit mAb #2118.
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue (1), GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis (2), gene expression (3), and nuclear transport (4). GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases (4,5).
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063 and U.S.S.N. 11/476,277) from Epitomics, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
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