规格:10U 等级:科研试剂 英文名称:Phosphodiesterase II from bovine spleen;3′-Exonuclease;Spleen exonuclease;Spleen phosphodiesterase 别名:磷酸二脂酶;磷酸二酯酶 CAS号:9068-54-6 级别:BR Activity:≥10u/mg 活力定义:One Unit increases the absorbance at 260 nm by 0.200 in 30 minutes at 37℃, pH 6.5, with an RNA substrate 产品描述:Spleen exonuclease (Phosphodiesterase II) excises 3"-phosphomononucleotides from oligonucleotides having a free OH terminus. The optimum pH for the enzyme is 5.5 using succinate and phosphate buffer and pH 6-7 with 0.1 M acetate buffer. The enzyme is assayed using a modification of the Hilmoe (Biochem. Prep., VII, (Meister, A., ed.), John Wiley and Sons, NY, 105, 1961) technique 外观:粉末 用途:生化研究。 保存:-20℃