Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) F=Flow Cytometry Reactivity Key: M=Mouse Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
DRAK2 (33D7) Rabbit mAb detects endogenous levels of total DRAK2 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surroundingAsp315 of mouse DRAK2.
Western Blotting
Western blot analysis of total cell lysates from BaF3 and mouse thymocytes, using DRAK2 (33D7) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse thymus, using DRAK2 (33D7) Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of BaF3 cells, using DRAK2 (33D7) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
Background
DRAK2 (DAP kinase related apoptosis inducing protein kinase 2) is a member of the novel DAP (death associated protein) pro-apoptotic kinase family (1). Overexpression of DRAK2 in NIH/3T3 cells induces morphological changes associated with apoptosis, which are likely to occur in a p53-dependent manner (1,2). DRAK2 is preferentially expressed in lymphoid tissues and regulates the TCR activation threshold during thymocyte selection (3). Indeed, T cells from DRAK2(-/-) mice exhibit enhanced sensitivity to T cell receptor-mediated stimulation and have a reduced requirement for co-stimulation (4).