HER2/ErbB2 (29D8) Rabbit mAb

价格询价

品牌Cell Signaling Technology
货号
2165
产地USA
更新日期2025年08月13日

应用范围W, IP, IHC-P, IHC-F, IF-IC, F
适应物种H,M,R

美国CST中国公司(丁香通商铺)

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详细信息
询价记录
文献和实验
技术资料

抗体英文名：
HER2/ErbB2 (29D8) Rabbit mAb
抗原：
synthetic peptide corresponding to residues surrounding tyrosines 1248 of human ErbB2 protein
应用范围：
W, IP, IHC-P, IHC-F, IF-IC, F
适应物种：
H,M,R
保质期：
详见说明书
库存：
大量
供应商：
CST
级别：
详见MSDS文件
是否单克隆：
1
保存条件：
-20°c
规格：
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity

规格：		产品价格：	￥请询价
规格：	40 ul (4 western blots)	产品价格：	￥请询价
规格：	100 ul (10 western blots)	产品价格：	￥请询价
规格：	carrier free & custom formulation / quantity	产品价格：	￥请询价

pathway more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IP IHC-P IHC-F IF-IC F	H (M) (R)	Endogenous	185 kDa	Rabbit IgG

Protocols	2165: Flow , IHC / Frozen , IHC / Paraffin , Immunofluorescence* , Immunoprecipitation , Western Blotting * Product-specific protocol.
Specificity / Sensitivity	HER2/ErbB2 (29D8) Rabbit mAb detects endogenous levels of total ErbB2 protein. This antibody does not cross-react with related kinases.
Source / Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding tyrosines 1248 of human ErbB2 protein. Western Blotting Western blot analysis of cell extracts from various cell lines, using HER2/ErbB2 (29D8) Rabbit mAb. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using HER2/ErbB2 (29D8) Rabbit mAb. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human breast carcinoma using HER2/ErbB2 (29D8) RmAb in the presence of control peptide (left) or HER2/ErbB2 Blocking Peptide #1059 (right). IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded SKBr3 (high HER2) (left), MDA-MB-453 (moderate HER2) (middle) and MDA-MB-468 (low HER2) (right), using HER2/ErbB2 (29D8) Rabbit mAb. IHC-F (frozen) Immunohistochemical analysis of frozen SKOV-3 xenograft using HER2/ErbB2 (29D8) Rabbit mAb. Flow Cytometry Flow cytometric analysis of MCF7 cells using HER2/ErbB2 (29D8) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red). IF-IC Confocal immunofluorescent analysis of MCF7 cells labeled with HER2/ErbB2 (29D8) Rabbit mAb (red). Blue= DRAQ5™ fluorescent DNA dye.
Background	The ErbB2 (HER2) proto-oncogene encodes a 185 kDa transmembrane, receptor-like glycoprotein with intrinsic tyrosine kinase activity (1). While ErbB2 lacks an identified ligand, ErbB2 kinase activity can be activated in the absence of a ligand when overexpressed and through heteromeric associations with other ErbB family members (2). Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers (3). Binding of the c-Cbl ubiquitin ligase to ErbB2 at Tyr1112 leads to ErbB2 poly-ubiquitination and enhances degradation of this kinase (4). ErbB2 is a key therapeutic target in the treatment of breast cancer and other carcinomas and targeting the regulation of ErbB2 degradation by the c-Cbl-regulated proteolytic pathway is one potential therapeutic strategy. Phosphorylation of the kinase domain residue Tyr877 of ErbB2 (homologous to Tyr416 of pp60c-Src) may be involved in regulating ErbB2 biological activity. The major autophosphorylation sites in ErbB2 are Tyr1248 and Tyr1221/1222; phosphorylation of these sites couples ErbB2 to the Ras-Raf-MAP kinase signal transduction pathway (1,5). Muthuswamy, S.K. et al. (1999) Mol Cell Biol 19, 6845-57. Qian, X. et al. (1994) Proc Natl Acad Sci USA 91, 1500-4. Dittadi, R. and Gion, M. (2000) J Natl Cancer Inst 92, 1443-4. Klapper, L.N. et al. (2000) Cancer Res 60, 3384-8. Kwon, Y.K. et al. (1997) J Neurosci 17, 8293-9.
Application References	Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
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