- ￥5300
- GeneTex
- 美国
- GTX88015
- 2025年09月22日
- ICC/IF, IHC-P
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
The antiserum was produced against synthesized peptide derived from human Histone H2BK5ac (1-50).
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX88015
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
ICC/IF, IHC-P
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
Histone H2BK5ac (acetyl Lys5)
- 抗体英文名：
Histone H2BK5ac (acetyl Lys5) antibody
- 抗体名：
Histone H2BK5ac (acetyl Lys5) 抗体
- 规格：
100 μg
ICC/IF analysis of HeLa cells using GTX88015 Histone H2BK5ac (acetyl Lys5) antibody. The picture on the right is blocked with the synthesized peptide.
IHC-P analysis of human breast carcinoma tissue using GTX88015 Histone H2BK5ac (acetyl Lys5) antibody. The picture on the right is blocked with the synthesized peptide.
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文献和实验Histone Deacetylase Activity Assay
Histone deacetylases (HDACs) are enzymes that catalyze the removal of acetyl groups from the ɛ-amino groups of conserved lysine residues in the amino terminal tail of histones. In humans, there are 18 potential deacetylase enzymes
组蛋白是和染色体相联的最主要的蛋白质。它的作用是和染色体中的 DNA 的负电荷结合。组蛋白是比较小的碱性蛋白；在细胞正常 pH 值时，组蛋白带有正电荷，这样它们就可以和 DNA 结合，这个正电荷主要存在于碱性氨基酸 Lys 和 Arg 的 -NH3 上。其实，组蛋白约含 25% 的 Arg 和 Lys 。比其他蛋白的 Arg ， Lys 的含量都多。和真核 DNA 结合的有 5 种类型的组蛋白： H1 ， H2A ， H2B ， H3
PROTOCOL To 1.5 mL eppendorf tubes add: 200 µg of protein extract (see Western blot protocol for protein sample preps) q.s. to 300 µL with RIPA (with protease and phosphatase inhibitors). Primary antibody (amount determined
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