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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Human recombinant protein fragment corresponding to amino acids 296-442 of human GATA4 (NP_002043) produced in E.coli.
- 亚型:
IgG3
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX84460
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IHC-P
- 浓度:
0.17 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
GATA4
- 抗体英文名:
GATA4 antibody [9F9]
- 抗体名:
GATA4 抗体 [9F9]
- 规格:
100 μl
WB analysis of various cell lines using GTX84460 GATA4 antibody [9F9].
Loading : 35 ug per lane
WB analysis of HEK293T cells transfected with GATA4 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84460 GATA4 antibody [9F9].
Loading : 5 ug per lane
ICC/IF analysis of COS7 cells transiently transfected with GATA4 plasmid using GTX84460 GATA4 antibody [9F9].
ICC/IF analysis of HeLa cells using GTX84460 GATA4 antibody [9F9].
IHC-P analysis of human endometrium tissue using GTX84460 GATA4 antibody [9F9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
IHC-P analysis of human pancreas carcinoma tissue using GTX84460 GATA4 antibody [9F9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
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文献和实验confirmation. A. Protein microarray hybridization with 8F1. The OriGene overexpression protein microarray chip was immunostained with the most commonly used 8F1 monoclonal anti-ERCC1 antibody. The positive reactive proteins are highlighted with red arrows
多篇 SCI 发现用 RIPA 提蛋白存在问题,我们该如何应对?
相互作用)及其他成分。虽然 RIPA 裂解液经过不断改良使用,但是 RIPA 提取蛋白通常会产生两个不同的部分:即 RIPA 可溶组分和 RIPA 不可溶组分。 一般来说,只有 RIPA 可溶组分被用于下游实验,而 RIPA 不可溶组分被丢弃。这样提取的蛋白在使用中会存在哪些问题呢?先来看些文献数据:研究发现,不同的蛋白在 RIPA 裂解液中溶解性不同,例如 F9 中的纤维连接蛋白不溶于 RIPA 裂解液 [2] 。而小鼠额叶样品中在 RIPA 可溶组分和非可溶性组分中含有相同量的 Septin
300, ETS1, ETS2, FKBP1B, FLJ14639 (NIP45), FOS, FOSL1, FOXO1A, FOXO3A, GATA3, GATA4, GRLF1, ICOS, IRF1, ISGF3G, JUN, JUNB, MADH4, MAPKAPK2, MAPKAPK3, MAX, MEF2A, MEF2B, MEF2C, MEF2D, MHC2TA, MKNK1, MLLT7, MYC, MYF5, NFKB2, NFKBIB, NFKBIE, NR4A2, PCNA, RAF
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