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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Human recombinant protein fragment corresponding to amino acids 1-150 and 428-527 of human ZIM2 (NP_056178) produced in E.coli.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX83403
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IHC-P, FACS
- 浓度:
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
ZIM2
- 抗体英文名:
ZIM2 antibody [5E12]
- 抗体名:
ZIM2 抗体 [5E12]
- 规格:
100 μl
IHC-P analysis of human endometrium tissue using GTX83403 ZIM2 antibody [5E12].
IHC-P analysis of human lymph node tissue using GTX83403 ZIM2 antibody [5E12].
WB analysis of HEK293T cells transfected with ZIM2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83403 ZIM2 antibody [5E12].
Loading : 5 ug per lane
IHC-P analysis of human lung carcinoma tissue using GTX83403 ZIM2 antibody [5E12].
IHC-P analysis of human breast adenocarcinoma tissue using GTX83403 ZIM2 antibody [5E12].
IHC-P analysis of human liver tissue using GTX83403 ZIM2 antibody [5E12].
IHC-P analysis of human ovary adenocarcinoma tissue using GTX83403 ZIM2 antibody [5E12].
FACS analysis of HEK293T cells transfected with either ZIM2 plasmid(Red) or empty vector control plasmid(Blue) using GTX83403 ZIM2 antibody [5E12].
ICC/IF analysis of COS7 cells transiently transfected with ZIM2 plasmid using GTX83403 ZIM2 antibody [5E12].
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文献和实验b-Gal Staining of Murine Embryos
to make 0.2% Glutaraldehyde fixative, eg: add 0.2ml 25% Glutaraldehyde (EM grade) into 24.8ml fixing buffer. (fresh make every time) For E10 - E12 embryo - fix the embryo on ice for 10 minutes (with at least 20ml fixative); - rinse with PBS
, with or without accentuation beneath the cell membrane or in paranuclear region.- This antibody stains stratified squamous epithelium and squamous cell carcinomas particularly well.- Major applications:a) 34 E12 stains the basal cells of the prostate, i.e. normal/reactive
In Situ Hybridization Protocols
. Column is now ready for use. D: Pre-absorbing Anti-Digoxygenin Antibody Fix a series of rat/chick E12.5 - E13.5 embryos in 4% paraformaldehyde for 2 hours at room temperature. Use about 8 rat embryos for every ml of 1:200 antibody. Wash
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