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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified recombinant fragment of human JAK3 expressed in E. Coli.
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX83303
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, FACS, ELISA
- 靶点:
JAK3
- 抗体英文名:
JAK3 antibody [5H2]
- 抗体名:
JAK3 抗体 [5H2]
- 规格:
100 μl
ICC/IF analysis of HeLa (left) and HepG2 (right) cells using GTX83303 JAK3 antibody [5H2].
Green : JAK3
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments
ICC/IF analysis of 3T3-L1 cells using GTX83303 JAK3 antibody [5H2].
Green : JAK3
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments
FACS analysis of HeLa cells using GTX83303 JAK3 antibody [5H2].
Blue : JAK3
Red : negative control
WB analysis of Jurkat cell lysate using GTX83303 JAK3 antibody [5H2].
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文献和实验相关专题 白介素21在自体免疫 和癌症中扮演的角色。Role of IL-21 in autoimmunity and cancer. IL-21 is involved in reciprocal cross talk between disparate cell types, leading to effects on innate immune responses and adaptive antibody
You will need the following reagents:- Antibodies Normal Swine Serum (NSS), 1:5 in Tris buffer. 1°: antibody, diluted in 1/20 NSS/Tris. 2° antibody, diluted in 1/20 NSS/Tris. Streptavidin/biotin complex StreptABComplex HRP
【共享】 蛋白质含量测定法/蛋白质沉淀法/Western免疫印迹
需要,标准蛋白质还可预先用微量凯氏定氮法测定蛋白氮含量,计算出其纯度,再根据其纯度,称量配制成标准蛋白质溶液。牛血清清蛋白用H2O 或0.9%NaCl配制,酪蛋白用0.05N NaOH配制。(2)双缩脲试剂:称以1.50克硫酸铜(CuSO4•5H2O)和6.0克酒石酸钾钠(KNaC4H4O6•4H2O),用500毫升水溶解,在搅拌下加入300毫升10% NaOH溶液,用水稀释到1升,贮存于塑料瓶中(或内壁涂以石蜡的瓶中)。此试剂可长期保存。若贮存瓶中有黑色沉淀出现,则需要重新配制。2. 器材:可见光分光
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