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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Preparation of chicken gizzard actin
- 亚型:
IgG1
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Chicken
- 目录编号:
GTX82740
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Mouse
- 应用范围:
WB, ICC/IF, IHC
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
Actin
- 抗体英文名:
Actin antibody [C4]
- 抗体名:
Actin 抗体 [C4]
- 规格:
100 μl
Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~42k actin protein using Actin antibody [C4] (GTX82740).
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文献和实验补体成分 C4(C4a/C4b) 补体成分:C4 血清浓度(μg/ml):200~500 分子量(kDa):210 亚单位(链)及分子量(kDa):α:90β:78γ:33 激活产物:C4a;C4b 生物学活性:C4a 弱的过敏毒素作用 C4b 组成CP中的C3、C5转化
the column with 3 l of Buffer D using a fast flow rate. 2. Elute the column with a 4 l gradient from 100 mM KCl to 500 mM KCl in Buffer D. Collect 25 ml fractions (400 drops/fraction). Actin-containing fractions are identified by dot blot using mAb C4
. For primary antibody use anti-GST at 1:1000 and anti-actin at 1:1000. For secondary use HRP conjugated IgG at 1:10,000. Actin Capture Assay Reagants Binding Buffer: 1X PBS pH 7.2 (Lane Recipe) + 10% Glycerol + 1mMMgCl2 + 0.2mM ATP + 0.1% BSA
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