- ￥4700
- GeneTex
- 美国
- GTX78443
- 2025年07月06日
- WB, ELISA, Functional Assay
- Mouse
- Human Immunodeficiency virus
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Bacterially expressed full-length HIV-1 protease
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human Immunodeficiency virus
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human Immunodeficiency virus
- 目录编号：
GTX78443
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ELISA, Functional Assay
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody recognizes free N-terminus of mature HIV protease (HIV-1 and HIV-2). The antibody 1696 does not react with the precursor.
- 抗体英文名：
HIV protease antibody [1696] (Azide Free)
- 抗体名：
HIV protease 抗体 [1696] (Azide Free)
- 规格：
100 μg
Dot Blot analysis of recombinant HIV protease. The total amount of recombinant HIV-protease spotted on the nitrocellulose membrane are indicated in left column.
Lane 1: anti-HIV protease (1696) (GTX78443); 0.2 μg/ml
Lane 2: anti-HIV protease (1696) (GTX78443); 1.0 μg/ml
Lane 3: anti-HIV protease (1696) (GTX78443); 2.0 μg/ml
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文献和实验Purification of Endotoxin Free mAbs
pressure of circulation to force water out through the membrane, and rapidly concentrating the antibody/proteins up to 100 fold. The advantage of this method is very large volumes can be processed using sterile and endotoxin free components. Ammonium
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
more details about inhibitor-protease binding than crystallography and isothermal titration calorimetry. The latest FDA-approved HIV-1 protease inhibitor is Darunavir (DRV). Herein, each DRV atom is evaluated by free energy component analysis
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