- ￥5000
- GeneTex
- 美国
- GTX78237
- 2025年07月14日
- WB, ICC/IF, IHC-P, FACS, IP, ELISA
- Mouse
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Cytoskeleton preparation of epidermal carcinoma cell line A431.
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
- 克隆性：
Monoclonal
- 标记物：
Biotin
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX78237
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, FACS, IP, ELISA
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Cytokeratin 18
- 抗体英文名：
Cytokeratin 18 antibody [C-04] (Biotin)
- 抗体名：
Cytokeratin 18 抗体 [C-04] (Biotin)
- 规格：
100 μg
WB analysis of HeLa cell lysate using GTX78237 Cytokeratin 18 antibody [C-04] (Biotin).
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文献和实验De Matteis R et al., Stem Cells 2009 (PMID:19688834)
Biotin labeling and hybridization of probe to chromosomes
quality) at 70 C for 2 min. Immediately transfer the slides into an ethanol series (70 %, 95 %, and 100 %, 5 min each at 20 C), and air dry the slides. Add 10 µl of the hybridization mixture to each slide and cover with an '18 x 18' mm
Preparation and purification of biotin-labeled probe for ISH
is collected in the 1.5 ml tube The final volume of probe should be close to 55 µl. Usually, 5 µl of such a biotin-labeled probe (1 µg/55 µl = 18 ng/µl) is enough for one slide, and the probe can be used to process 100 slides. The size of the probe
in detail here. The first protocol is essentially a dual-pulldown assay employing Flag-tagged DNMT3A or antibody of choice for an endogenous protein and 5' biotin linked antisense RNA (Figure 1a), while the second protocol is a triple pulldown assay which essentially expands
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