- ￥1700 - 4000
- GeneTex
- 美国
- GTX633420
- 2025年07月16日
- WB, ICC/IF, IHC-P
- Mouse
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Carrier-protein conjugated synthetic peptide surrounding acetyl Lys8 of human Histone H4. The exact sequence is proprietary.
- 亚型：
IgG3
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX633420
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P
- 浓度：
0.95 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Histone H4K8ac (acetyl Lys8)
- 抗体英文名：
Histone H4K8ac (acetyl Lys8) antibody [GT478]
- 抗体名：
Histone H4K8ac (acetyl Lys8) 抗体 [GT478]
- 规格：
100 μl/25 μl
| 规格： | 100 μl | 产品价格： | ￥4000.0 |
|---|---|---|---|
| 规格： | 25 μl | 产品价格： | ￥1700.0 |
Histone H4K8ac (acetyl Lys8) antibody [GT478] detects Histone H4K8ac (acetyl Lys8) protein at cytoplasm and nucleus in rat kidney by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Histone H4K8ac (acetyl Lys8) antibody [GT478] (GTX633420) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Histone H4K8ac (acetyl Lys8) antibody [GT478] detects Histone H4K8ac (acetyl Lys8) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: Histone H4K8ac (acetyl Lys8) protein stained by Histone H4K8ac (acetyl Lys8) antibody [GT478] (GTX633420) diluted at 1:400.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody (GTX102078) diluted at 1:300.
Scale bar = 10 μm.
Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H4K8ac (acetyl Lys8) antibody (GTX633420) diluted at 1:1000.
Histone H4K8ac (acetyl Lys8) antibody [GT478] detects Histone H4K8ac (acetyl Lys8) protein at nucleus in human ovary by immunohistochemical analysis.
Sample: Paraffin-embedded human ovary.
Histone H4K8ac (acetyl Lys8) antibody [GT478] (GTX633420) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Histone H4K8ac (acetyl Lys8) antibody [GT478] detects Histone H4K8ac (acetyl Lys8) protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Histone H4K8ac (acetyl Lys8) antibody [GT478] (GTX633420) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献和实验Samata M et al., Cell 2020 (PMID:32502394)
，为此CST公司推出了可用于蛋白功能检测的乙酰化组蛋白系列抗体,圆满的解决这个问题。因为乙酰化与蛋白的活性相关，因此通过乙酰化的系列抗体特异性地识别靶蛋白的乙酰化形式，可以反映出靶蛋白质的活性水平，达到研究蛋白功能和调节的目的。而其他的抗体仅能反映出该蛋白质的表达量或表达水平，不能反映出蛋白的活性状态。乙酰化系列抗体：Catalog#Product DescriptionSourceApplications2576Acetyl-Histone H2A (Lys5) AntibodyRabbitW IP
Identifying Acetylated Proteins in Mitosis
the acetylation status of several mitotic proteins by anti-acetyl-Lysine immunoprecipitation with a monoclonal antibody followed by Western blot analyses of the proteins of interest. We further confirmed by a reciprocal immunoprecipitation with protein-specific
Synthesis and Probing of Membrane-bound Peptide Arrays
should be Cys(Acm) or Cys(Trt), Asp(OtBu), Glu(OtBu), His(Trt), Lys(Boc), Asn(Trt), Gln(Trt), Arg(Pmc), Ser(tBu), Thr(tBu), Trp(Boc), and Tyr(tBu). HOBt-esters of these amino acid derivatives must be prepared in NMP for use throughout in spotting reactions
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