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SARS-CoV / SARS-CoV-2 (COVID-1

9) spike antibody [1A9]
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  • ¥1700 - 4000
  • GeneTex已认证
  • 美国
  • GTX632604
  • 2025年07月14日
  • WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, EM, Neutralizing/Inhibition, Sandwich ELISA, IHC-P (cell pellet)
  • Mouse
  • SARS Coronavirus, SARS Coronavirus 2
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      The immunogen used to generate this antibody corresponds to SARS-CoV SΔ10 (within S2 domain) protein (1029-1192 a.a.). (SARS-CoV strain: Sin2774) The exact sequence is proprietary.

    • 亚型

      IgG1

    • 形态

      Liquid

    • 保存条件

      Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4℃. For long-term storage, aliquot and store at -20℃ or below. Avoid multiple freeze-thaw cycles.

    • 克隆性

      Monoclonal

    • 标记物

      Unconjugated

    • 适应物种

      SARS Coronavirus, SARS Coronavirus 2

    • 保质期

      12 months from the shipping date of the product. 

    • 抗原来源

      SARS Coronavirus

    • 目录编号

      GTX632604

    • 级别

      Primary Antibodies

    • 库存

      Available

    • 供应商

      GeneTex

    • 宿主

      Mouse

    • 应用范围

      WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, EM, Neutralizing/Inhibition, Sandwich ELISA, IHC-P (cell pellet)

    • 浓度

      1 mg/ml(Please refer to the vial label for the specific concentration.)

    • 靶点

      Our internal testing indicates no cross-reactivity with MERS-CoV spike protein.This antibody is able to detect multiple SARS-CoV-2 VOCs, including Omicron variant.

    • 抗体英文名

      SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9]

    • 抗体名

      SARS-CoV / SARS-CoV-2 (COVID-19) spike 抗体 [1A9]

    • 规格

      100 μl/25 μl

    规格:100 μl产品价格:¥4000.0
    规格:25 μl产品价格:¥1700.0
    产品细节图片1

    Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, Omicron / BA.2.12.1 variant, His tag (GTX137114-pro) (28.62-0.45 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

    产品细节图片2

    SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] detects SARS-CoV-2 (COVID-19) spike protein by immunofluorescent analysis.
    Sample: BHK-21 cells transfected with full-length SARS-CoV-2 spike were fixed in 4% PFA at RT for 30 min.
    Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:2000.

    产品细节图片3

    Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using GTX632604 as capture antibody at concentration of 5 μg/mL and GTX635672 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

    产品细节图片4

    Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 spike (ECD) trimer, omicron BA.2.75 variant, His tag (GTX137533-pro) (28.62-0.45 nM). Coated protein was probed with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (the specified SARS-CoV-2 (COVID-19) Spike antibodies) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.

    产品细节图片5

    Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

    产品细节图片6

    Immunoprecipitation of SARS-CoV-2 Spike transfected 293T whole cell extracts using 2 μg of SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604).
    Western blot analysis was performed using SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604).
    EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01) was used to detect the primary antibody.

    产品细节图片7

    Indirect ELISA analysis was performed by coating the plate with recombinant SARS-CoV-2 (COVID-19) Spike (ECD) Protein, BA.2 / Omicron variant, His tag (GTX137037-pro) (29.71-0.46 nM). Coated protein was probed with the specified SARS-CoV-2 (COVID-19) Spike antibodies (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) or goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) were used to detect the bound primary antibodies.

    产品细节图片8

    Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 spike (full length) transfected (GTX535664) 293T whole cell extracts using GTX632604 as capture antibody at concentration of 5 μg/mL and GTX135356 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

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    图标文献和实验
    该产品被引用文献
    Application Reference
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    相关实验
    • SARS-CoV-2 抗原检测: 检测安全性探讨

      COVID-19 infection. Nature Scientifc Reports 2:MacMullan,MA et al. (2020) ELISA detection of SARS-CoV-2 antibodies in saliva. Nature Scientifc Reports 3:Hettegger,P et al. (2019) High similarity of IgG antibody profles in blood and saliva opens

    • Cell Death Differ:中大五院黄曦团队发现新冠病毒引起肺损伤的分子机制

      近期,中山大学附属第五医院黄曦团队报道了 SARS-CoV-2 的 membrane (M) 蛋白通过靶向并抑制胞内 BOK 的泛素化降解,激活肺上皮细胞线粒体凋亡途径而引起肺损伤,该发现为 COVID-19 的靶向治疗提供了潜在靶点。 图片来源:Cell death & differentiation SARS-CoV-2 感染已经导致全球数以百万人的死亡,其中大部分死亡患者出现呼吸困难,肺部出现大量凋亡的细胞和渗漏的粘液,已有研究证实 SARS-CoV-2 感染引起肺细胞凋亡参与上述

    • 中科院高福院士课题组 2020 年发表了哪些重要的研究成果?

      :Science 9、Science 后再发 Nature!筛选出又一新冠病毒治疗性单克隆中和抗体DOI: 10.1038/s41586-020-2381-y继之前发表在 Science 上报道筛选出新冠病毒中和抗体后,2020 年 5 月 26 日,高福院士再次联合严景华等人在 Nature 发文:A human neutralizing antibody targets the receptor-binding site of SARS-CoV-2。该研究报告了从 COVID-19 恢复期的患者中分离出两

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    文献支持
    SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9]
    ¥1700 - 4000