- ￥2300
- GeneTex
- 美国
- GTX628461-01
- 2025年07月14日
- WB
- Mouse
- Rat
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- 详细信息
- 文献和实验
- 技术资料
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
HRP
- 适应物种：
Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Rat
- 目录编号：
GTX628461-01
- 级别：
Secondary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB
- 浓度：
0.22 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
Mouse Anti-Rat IgG (Light chain)
- 抗体英文名：
Mouse Anti-Rat IgG (Light chain) antibody [GT569] (HRP)
- 抗体名：
Mouse Anti-Rat IgG (Light chain) 抗体 [GT569] (HRP)
- 规格：
250 μl
Rat IgG were separated by 12% SDS-PAGE, and the membrane was blotted with Rat IgG (Light chain) antibody [GT569] (HRP) (GTX628461-01) diluted at 1:1000.
Mouse anti-Rat IgG (light chain) antibody [GT569] (HRP) detects Rat IgG (light chain) protein by Western blot analysis.
A. 0.4 μg native rat IgG
B. 8 μg denature rat IgG
10 % SDS-PAGE
Mouse anti-Rat IgG (light chain) antibody [GT569] (HRP) (GTX628461-01) dilution: 1:1000
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文献和实验Shabnam Shalapour et al., Nature 2015 (PMID:25924065)
Cloning and Expression of Single-Chain Fragments (scFv) from Mouse and Rat Hybridomas
information for VH and VL is generally amplified from hybridoma cells using the polymerase chain reaction (PCR) with antibody-specific primers A variety of primer sets for the amplification of mouse-variable domams has been developed that are particulary
1 μL 5) Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC™ capture beads (Component A in the AbC™ Anti-Mouse Bead Kit) to each tube. 6)
IMMUNOSCREENING OF LAMBDA-GT11 BACTERIOPHAGE LIBRARIES WITH ANTIBODY PROBES
dishes of lysed E. coli to reduce anti-E. coli antibody background. These antibodies are present in all human sera, rabbit sera, and rodent sera. For each Petri dish containing solidified LB agar, mix 0.6ml of the Y1090 overnight-saturated culture with 30
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