- ￥6300
- GeneTex
- 美国
- GTX60885
- 2025年07月14日
- WB, ICC/IF, ELISA, ChIP assay, IHC, Sandwich ELISA
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys9).
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX60885
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, ELISA, ChIP assay, IHC, Sandwich ELISA
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with other acetylated Lysines in histone H3.
- 抗体英文名：
Histone H3K9ac (Acetyl Lys9) antibody [RM161]
- 抗体名：
Histone H3K9ac (Acetyl Lys9) 抗体 [RM161]
- 规格：
100 μg
WB analysis of acid extracts from HeLa cells untreated (-) or treated with sodium butyrate (+) using GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161].
Dilution : 0.25μg/ml
ChIP analysis of HeLa cells using GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161]. Real-time PCR was performed using primers specific to the gene indicated.
Antibody amount : 5μg
ICC/IF analysis of HeLa cells treated with sodium butyrate using GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161].
Red : Primary antibody
Green : Actin
Sandwich ELISA analysis of HeLa cell treated or untreated with Sodium Butyrate lysates using GTX60888 Histone H3 antibody [RM188] (1 ug/mL) as the capture antibody and biotinylated GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161] (1 ug/mL) as the detection antibody. Please notice that GTX60885 needs to be conjugated to Biotin to function as the detection antibody when paired with GTX60888. Biotin-conjugated form is not provided.
ICC/IF analysis of HepG2 cells using GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161].
The GTX60885 reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with acetyl-ated Lysine 4 (K4ac), Lysine 14 (K14ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac), Lysine 79 (K79ac), or Lysine 122 (K122) in histone H3.
Sandwich ELISA analysis of HeLa cell treated or untreated with Sodium Butyrate lysates using biotinylated GTX60885 Histone H3K9ac (Acetyl Lys9) antibody [RM161] (1 ug/mL) as the detection antibody and GTX60888 Histone H3 antibody [RM188] (1 ug/mL) as the capture antibody. Please notice that GTX60885 needs to be conjugated to Biotin to function as the detection antibody when paired with GTX60888. Biotin-conjugated form is not provided.
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文献和实验) AntibodyRabbitW IHC IC9671Acetyl-Histone H3 (Lys9) AntibodyRabbitW IP IHC IC2591Acetyl-Histone H4 (Lys12) AntibodyRabbitW IP IHC IC2594Acetyl-Histone H4 (Lys8) AntibodyRabbitW IP IHC IC有时组蛋白同时乙酰化和磷酸化修饰，进行转录的调控和活性的调节，因此CST也推出了能同时检测乙酰化和磷酸化的组蛋白的抗体。该系列抗体只识别同时乙酰化和磷酸
Identifying Acetylated Proteins in Mitosis
the acetylation status of several mitotic proteins by anti-acetyl-Lysine immunoprecipitation with a monoclonal antibody followed by Western blot analyses of the proteins of interest. We further confirmed by a reciprocal immunoprecipitation with protein-specific
from the antibody-bound and antibody-unbound fractions (from Chromatin Immunoprecipitation on Unfixed Chromatin from Cells and Tissues to Analyze Histone Modifications). Control genomic DNA should be used as well. For each PCR reaction, we use 20-50 ng of template
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