- ￥5000
- GeneTex
- 美国
- GTX55091
- 2025年10月24日
- WB
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
KLH-conjugated synthetic peptide encompassing a sequence within the center region of C/EBP alpha. The exact sequence is proprietary.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX55091
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
C/EBP alpha (phospho Thr226)
- 抗体英文名：
C/EBP alpha (phospho Thr226) antibody
- 抗体名：
C/EBP alpha (phospho Thr226) 抗体
- 规格：
100 μl
WB analysis of EGF-treated MCF7 (A), mouse spleen (B), mouse liver (C), rat spleen (D), rat liver (E) whole cell lysates using GTX55091 C/EBP alpha (phospho Thr226) antibody.
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
In situ hybridisation to alpha satellite sequences (chromosome specific)
can be done without formamide at higher temperature (try 0.25 x SSC at 72C for 5 minutes) Formamide wash solution: 60ml formamide TOXIC (Sigma F7503), 30ml H2 O, 10ml 20 x SSC, 80 m l conc. HCl (to bring the pH to 7) Do not allow the slides to dry at any stage
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
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