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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of NFkB p65. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX54983
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
NFkB p65 (phospho Thr435)
- 抗体英文名:
NFkB p65 (phospho Thr435) antibody
- 抗体名:
NFkB p65 (phospho Thr435) 抗体
- 规格:
100 μl
IHC-P analysis of formalin fixed human breast cancer tissue section using GTX54983 NFkB p65 (phospho Thr435) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)
ICC/IF analysis of formalin-fixed K562 cells using GTX54983 NFkB p65 (phospho Thr435) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
WB analysis of TNFa-treated Jurkat (A), TNFa-treated HepG2 (B) whole cell lysates using GTX54983 NFkB p65 (phospho Thr435) antibody.
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文献和实验sakura042 本新手欲研究nfkb引起内皮细胞凋亡的机制:是否通过jnk通路。 这是否是一个信号通路串话的问题? 我们用特异性抑制剂阻断nfkb后发现jnk活性下降,是否就能说明nfkb促进内皮细胞凋亡是通过jnk通路呢?还需要再阻断或者过表达jnk通路来反证吗? 这个问题困扰了我好久,请教各位高手。 sakura042 自己顶一下 三叶虫 请问一下
【求助】矛盾的实验结果:IKb alpha和NFKB同时升高
吴佰霖 我的基因过表达,结合文献,推理应该是IKb alpha下降,而NFKB升高 (IKb可磷酸化NFKB,使后者降低) 但现在我的结果是两者同时升高,请问可能的原因有哪些呢? 谢谢 woxingwosu 可否检测一下NFKB的磷酸化情况? erik NFKB是检测核内的吗?检测p65还是p50/ 吴佰霖 检测总蛋白
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
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