- ￥5300
- GeneTex
- 美国
- GTX54522
- 2025年07月15日
- WB, ICC/IF, IHC-Fr, ELISA
- Rabbit
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Synthetic peptide conjugated to KLH via cysteine corresponding to CPSDSSEIVLD (577-586) of Human HTT.
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX54522
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-Fr, ELISA
- 浓度：
0.6 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
This antibody recognizes the 586 cleaved fragment without detecting the full-length form.
- 抗体英文名：
Huntingtin (neoepitope 586) antibody
- 抗体名：
Huntingtin (neoepitope 586) 抗体
- 规格：
100 μl
Sandwich ELISA was performed with a Huntingtin monoclonal antibody and GTX54522 Huntingtin (neoepitope 586) antibody to determine the antigen concentration of the Huntingtin cleavage products. The curve represents a dose response for neo586 in 293T cells overexpressing the Htt construct.
ICC/IF analysis of 293T cells transfected with Htt23Q and Htt148Q stop constructs ending in amino acid 586 using GTX54522 Huntingtin (neoepitope 586) antibody.
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
Dilution : 1:50 for at least 1 hour at room temperature
IHC-Fr analysis of mouse striatum (top panels) and cortex (bottom panels) tissue from either transgenic HD mice of the YAC128 line (left panels) or their WT littermates (right panels) using GTX54522 Huntingtin (neoepitope 586) antibody.
Green : Primary antibody
Dilution : 1:50
WB analysis of endogenous HTT lysates with or without different caspase activity (Lanes 1-5) and overexpressed recombinant HTT fragment lysates (Lanes 6-9) using GTX54522 Huntingtin (neoepitope 586) antibody (Bottom panel) and pan-HTT antibody (Top panel).
Dilution : 1:500
Loading :20 μg
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