ELISA detection of COMT using GTX101233 for capture at a concentration of 2.5 μg/mL and GTX89277 for detection at a concentration of 0.5 μg/mL. The HRP-conjugated anti-goat IgG antibody (GTX26741) was diluted at 1:20,000-1:200,000 and used to detect the primary antibody.