This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length bovine S100 protein (mixture of aa homodimers and ab heterodimers).
亚型:
IgG
形态:
Liquid
保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
克隆性:
Polyclonal
标记物:
Unconjugated
适应物种:
Human, Mouse, Rat, Bovine
保质期:
12 months from the shipping date of the product.
抗原来源:
Bovine
目录编号:
GTX48819
级别:
Primary Antibodies
库存:
Available
供应商:
GeneTex
宿主:
Rabbit
应用范围:
WB, IHC-P, ELISA
浓度:
5 mg/ml (Please refer to the vial label for the specific concentration.)
靶点:
S100
抗体英文名:
S100 antibody
抗体名:
S100 抗体
规格:
100 μg
Rabbit anti-S100 was used at a 1:500 dilution to detect S100 by immunohistochemistry in human brain astrocyte tumor tissue. Tissue was formalin-fixed and paraffin embedded.
Rabbit anti-S-100 protein was used at a 1:500 dilution to detect S-100 by immunohistochemistry using a 2-step indirect method. Dark nuclear staining is observed within basket cells located near the Purkinje cells in the cerebellum. Mouse brain tissue was immersed for 24 hours in 10% neutral buffered formalin and paraffin processed followed by sectioning at 4 microns. No antigen unmasking (HIER) or protease digestion was performed prior to immunostaining. Sections were deparaffinized in xylene, and hydrated through graded alcohol to distilled water. All incubations were done at room temperature. All rinses were either distilled water or Tris-HCl with 0.05% Tween 20. Endogenous peroxidase activity was blocked with 3% Hydrogen peroxide for 10'. Primary antibody was diluted as stated and reacted for 30' followed by washes and the addition of donkey anti-rabbit HRP diluted 1:500 for 30'. DAB+ (Dakocytomation) was used as a substrate and was allowed to react for 5'.
Western blot using GeneTex's Affinity Purified anti-S-100 antibody shows detection of a band ~11 kDa corresponding to bovine S-100 monomer (100 μg loaded, arrowhead lane 1). The antibody also detects S-100 from rat brain lysate (lane 2). Approximately 35 μg of a rat brain whole cell lysate was separated by 16% SDS-PAGE and transferred onto nitrocellulose. After blocking, the membrane was probed with the primary antibody diluted to 1:1,000 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated goat anti-Rabbit IgG [H&L] for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.