- ￥5000
- GeneTex
- 美国
- GTX48548
- 2025年07月11日
- WB, ICC/IF, IHC-P, FACS, IP, ChIP assay, MS, IHC (Free Floating)
- Mouse
- Human, Mouse, Rat, Pig
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
This antibody was raised against a synthetic peptide corresponding to amino acids 637-650 (EKDDREDKENAFKR) of human Dnmt1 (Genbank Accession No. NP_001370).
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat, Pig
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX48548
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, IHC-P, FACS, IP, ChIP assay, MS, IHC (Free Floating)
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
DNMT1
- 抗体英文名：
DNMT1 antibody [60B1220.1]
- 抗体名：
DNMT1 抗体 [60B1220.1]
- 规格：
50 μg
WB analysis of HeLa cell lysate using GTX48548 DNMT1 antibody [60B1220.1].
ICC/IF analysis of HeLa cells using GTX48548 DNMT1 antibody [60B1220.1].
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:10
FACS (Intracellular staining) analysis of HeLa cells using GTX48548 DNMT1 antibody [60B1220.1].
Blue : Primary antibody
Orange : isotype control
Fixation : 4% PFA
WB analysis of 2102EP cells / human DNMT1 recombinant protein / NIH3T3 cells / D3 cells in the (A)/(C)/(E)/(G) absence and (B)/(D)/(F)/(H) presence of immunizing peptide using GTX48548 DNMT1 antibody [60B1220.1].
Dilution : 5 μg/ml for cells / 0.1μg/ml for recombinant protein
IHC-P analysis of human hepatocellular carcinoma tissue using GTX48548 DNMT1 antibody [60B1220.1].
Dilution : 1:50
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文献和实验Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap
) (Abcam, Cambridge, UK; Sigma, St. Louis, USA.; Roche, Mannheim, Germany). We recently generated a GFP-binding protein (GBP) based on a single domain antibody derived from Lama alpaca (Rothbauer et al., 2008). This GFP-binding protein is characterized
antibody at 5 µg/ml for 45 min at 4 °C. Mouse anti-human NFB p65 monoclonal antibody sc-8008 (Santa Cruz, CA, USA) recognizes epitopes mapping the C amino-acid terminus of human origin NFB p65. After two washes in PBS–FCS, nuclei were incubated for 45 min
of First Hospital of Harbin Medical University. RPMI 1640 was purchased from GIBCO. Propidium iodide and Rnase were from Sigma Chemical Co. A murine monoclonal antibody against human Bcl-2 and Bax oncoprotein and antimi ce rabbit polyclonal antibody were purchased
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