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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Asparaginase (E. coli).
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
HRP
- 适应物种:
E. coli
- 保质期:
12 months from the shipping date of the product.
- 目录编号:
GTX40848
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ELISA
- 浓度:
10 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Recognises purified and partially purified Asparaginase (E. coli).
- 抗体英文名:
Asparaginase antibody (HRP)
- 抗体名:
Asparaginase 抗体 (HRP)
- 规格:
1 mg
Western Blot of Peroxidase conjugated Rabbit anti-L-ASPARAGINASE antibody(GTX40848). Lane 1: L-ASPARAGINASE. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase rabbit secondary antibody at 1:1,000 for 60 min at RT. Block for 30 min RT. Predicted/Observed size: 30 kDa for L-ASPARAGINASE. Other band(s): L-ASPARAGINASE splice variants and isoforms.
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文献和实验Hu Y et al., Biomaterials 2021 (PMID:33360506)
Maria Morales-Gonzalez et al., Tropical Journal of Pharmaceutical Research 2018;17(11)
Conjugation of Antibody to HRP
of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator. Desalt the HRP on PBS equilibrated column. Collect
Conjugation of Antibody to HRP
Conjugation of Antibody to HRP Author: Nanci Donacki Source: Contributed by Nanci Donacki Reagents
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
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