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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
A trimethyl-phospho-peptide corresponding to Trimethyl- Phospho-Histone H3 (Lys9/Ser10).
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX33602
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ELISA
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
This antibody reacts to Histone H3 only when modified by both trimethylation at lysine 9 and phosphorylation at serine 10 (K9me3/S10p).
- 抗体英文名:
Histone H3K9me3S10ph (Tri-methyl Lys9 / phospho Ser10) antibody [RM162]
- 抗体名:
Histone H3K9me3S10ph (Tri-methyl Lys9 / phospho Ser10) 抗体 [RM162]
- 规格:
100 μg
WB analysis of acid extracts of HeLa cells (1) andrecombinant histone H3.3 (2) using GTX33602 Histone H3K9me3S10ph (Tri-methyl Lys9 / phospho Ser10) antibody [RM162].
Dilution : 0.01μg/ml
The GTX33602 reacts to Histone H3 only when modified by both trimethylation at lysine 9 and phosphorylation at serine 10 (K9me3/S10p). Nocross reactivity with non-modified Lysine 9/ Serine 10,methylated Lysine 9 (K9me1, k9me2, k9me3) ONLY, or phosphorylation at Serine 9 ONLY in Histone H3.
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available
secondary antibody review -- data from 99 publications
IgG Alexa Fluor 488 1:250 Molecular Probes 10 goat Cy3 immunocytochemistry detect NMDA receptors in HEK293 cells Jackson ImmunoResearch Laboratories 10 FITC immunocytochemistry 1:100 detect phospho-CREB in human colonic
) 10% (w/v) ammonium persulfate (APS; prepare fresh for each gel) 10% Triton X‐100 (protein grade; Calbiochem)
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