- ￥5000
- GeneTex
- 美国
- GTX32541
- 2025年07月12日
- WB, ICC/IF, IHC-P, IP, ChIP assay
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CTCF (NP_006556.1).
- 亚型：
IgG
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Polyclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX32541
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Rabbit
- 应用范围：
WB, ICC/IF, IHC-P, IP, ChIP assay
- 浓度：
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点：
CTCF
- 抗体英文名：
CTCF antibody
- 抗体名：
CTCF 抗体
- 规格：
100 μl
IP analysis of HeLa cell lysate using GTX32541 CTCF antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000
WB analysis of various sample lysates using GTX32541 CTCF antibody.
Dilution : 1:1000
Loading : 25μg per lane
ChIP analysis of HCT116 cell lysate using GTX32541 CTCF antibody. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
IHC-P analysis of rat brain tissue using GTX32541 CTCF antibody.
Dilution : 1:100
IHC-P analysis of mouse brain tissue using GTX32541 CTCF antibody.
Dilution : 1:100
ICC/IF analysis of U2OS cells using GTX32541 CTCF antibody.
Blue : DAPI
Dilution : 1:100
ICC/IF analysis of HeLa cells using GTX32541 CTCF antibody.
Blue : DAPI
Dilution : 1:200
IHC-P analysis of human colon tissue using GTX32541 CTCF antibody.
Dilution : 1:100
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文献和实验Generation of Poly(ADP-ribosyl)ation Deficient Mutants of the Transcription Factor, CTCF
of the PARylation mark. The latter was achieved by ectopic expression of mutated proteins in cells, followed by immunoprecipitation with the polyclonal anti-PAR antibody and Western analysis with a protein-specific antibody. The PARylation-deficient CTCF mutant
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
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