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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of AMPK alpha 1. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX32345
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
AMPK alpha 1 (phospho Ser496)
- 抗体英文名:
AMPK alpha 1 (phospho Ser496) antibody
- 抗体名:
AMPK alpha 1 (phospho Ser496) 抗体
- 规格:
100 μl
IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32345 AMPK alpha 1 (phospho Ser496) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)
WB analysis of UV-treated HUVEC (A), HeLa (B), SP2/0 (C), PC12 (D) whole cell lysates using GTX32345 AMPK alpha 1 (phospho Ser496) antibody.
ICC/IF analysis of formalin-fixed HUVEC cells using GTX32345 AMPK alpha 1 (phospho Ser496) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
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文献和实验secondary antibody review -- data from 99 publications
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v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
3.1 and 3.6 ). This will provide a “specific activity” measurement relative to total protein in the cell, and is themost accurate measure of total GSK3 activity, or2. Immunoblot using antibodies to phospho-Ser21 (GSK3a)or phospho-Ser9 (GSK3b) (see Sections 3.1 –
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